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| PolysacDB ID | 1616 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Moraxella catarrhalis (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo[ branched to Kdop-(2-?)] |
| BCSDB Structure | 22728 |
| Proposed Function | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature | Whole cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 8E7 |
| Antibody Type Class | IgG3 |
| Assay System | ELISA, immunoblotting, Bacterial adherence assay, Bactericidal assay and immunoelectron microscopy |
| Cross Reactivity | MAb 8E7 reacted with M. catarrhalis O35E, serotype A and C LOSs but not serotype B LOS |
| Proposed Epitope | The epitope recognized by MAb 8E7 is directed to a common sequence of either α-GlcNAc-(1-->2)-β-Glc-(1--> at the branch substituting position 4 of the trisubstituted Glc residue or a terminal tetrasaccharide α-Gal-(1-->4)-β-Gal-(1-->4)-α-Glc-(1-->2)-β-Glc-[1--> at the branch substituting position 6 of the trisubstituted Glc residue |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab had had bactericidal activity. Passive immunization with Mab 8E7 could significantly enhance the clearance of strain O35E from mouse lungs in an aerosol challenge mouse model |
| Curator ID | AA + AS |
| Date of Curation | 23-09-2010 |
| References | PMC98028 |
| PolysacDB ID | 1617 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Moraxella catarrhalis serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo |
| BCSDB Structure | 22728 |
| Proposed Function | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature | Whole cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Polysera |
| Antibody Type Class | N/A |
| Assay System | ELISA and immunoblotting |
| Cross Reactivity | This antisera was specific to serotype A |
| Proposed Epitope | A terminal α-D-GlcNAc-(1-->2)-β-D-Glc seemed to be a part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera may help in sertotyping of moraxella strains |
| Curator ID | AA + AS |
| Date of Curation | 23-09-2010 |
| References | 8636949 |
| PolysacDB ID | 1618 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Moraxella catarrhalis serotype B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->4)-α-D-Glcp [branched to α-D-Galp-(1-->4)-&blpha;-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo |
| BCSDB Structure | 21752 |
| Proposed Function | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature | Whole cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Polysera |
| Antibody Type Class | N/A |
| Assay System | ELISA and immunoblotting |
| Cross Reactivity | This antisera was specific to serotype B |
| Proposed Epitope | A terminal β-D-Gal-(1-->4)-β-D-Glc seemed to be a part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera may help in sertotyping of moraxella strains |
| Curator ID | AA + AS |
| Date of Curation | 24-09-2010 |
| References | 8636949 |
| PolysacDB ID | 1619 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Moraxella catarrhalis serotype C (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to β-D-Galp-(1-->4)-α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-α-Kdop [branched to Kdop-(2-->4)] |
| BCSDB Structure | 22728 |
| Proposed Function | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature | Whole cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Polysera |
| Antibody Type Class | N/A |
| Assay System | ELISA and immunoblotting |
| Cross Reactivity | This antisera cross-reacted with Serotype A LPS |
| Proposed Epitope | A terminal β-D-Gal-(1-->4)-α-D-GlcNA seemed to be a part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera may help in sertotyping of moraxella strains |
| Curator ID | AA + AS |
| Date of Curation | 24-09-2010 |
| References | 8636949 |
| PolysacDB ID | 1620 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Moraxella catarrhalis B strain 26397 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->4)-α-D-Glcp [branched to α-D-Galp-(1-->4)-&blpha;-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo |
| BCSDB Structure | N/A |
| Proposed Function | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | Adipic acid dihydrazide [ADH] was introduced to the carboxyl group of Kdo moiety of the detoxified lipooligosaccharide [LOS] to form adipic hydrazide (AH)-dLOS derivatives, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) and N-hydroxysulfosuccinimid. dLOS (96 mg) was dissolved in 12 ml of 287 mM ADH (50 mg/ml, molar ratio of ADH to dLOS is 108 to 1 based on an estimated Mrof 3,000 for dLOS). AH-dLOS (45 mg) was dissolved with 4.5 ml of 0.2 M NaCl to make a 10 mg/ml solution of AH-dLOS. For the conjugation reaction, each 20 mg of AH-dLOS solution (2 ml in volume) was mixed with 10 mg of cross-reactive mutant (CRM) of diphtheria toxin (0.44 ml in volume). The initial concentration of AH-dLOS or CRM was 8.20 or 4.10 mg/ml. The molar ratio of AH-dLOS to CRM (Mr 67,000) was 45 to 1. The pH was adjusted to 5.0-5.2 with 0.1 M HCl, followed by addition of 0.05 M EDC. The reaction was maintained at pH 5.0 to 5.2 for 4 h at 4°C, then adjusted to pH 7.0, dialyzed against 0.9% NaCl for 2 to 3 days, centrifuged, and passed through a Sephacryl S-300 column (2.6 by 90 cm) in 0.9% NaCl. Peaks that contained both protein and carbohydrate were pooled and designated as dLOS-TT or dLOS-CRM. Both conjugates were analyzed for their composition of carbohydrate and protein using dLOS and bovine serum albumin (BSA) as standards |
| Antibodies | Polysera |
| Antibody Type Class | N/A |
| Assay System | ELISA, Bactericidal assay and immunoblotting |
| Cross Reactivity | This antisera was specific to Serotype B and reacted with nine of twelve clinical isolates studied |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera showed elevated complement-mediated bactericidal activity against the homologous strain |
| Curator ID | AA + AS |
| Date of Curation | 24-09-2010 |
| References | PMC1087343 |
| PolysacDB ID | 1621 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Moraxella catarrhalis Serotype A strain 25238 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)-(1-->5)-Kdo |
| BCSDB Structure | N/A |
| Proposed Function | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | Adipic acid dihydrazide (ADH) was bound to detoxified lipooligosaccharide [LOS] to form adipic hydrazide (AH)-dLOS derivatives, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) and N-hydroxysulfosuccinimide (sulfo-NHS). dLOS (70 mg) was dissolved in 7 ml of 345 mM ADH (molar ratio of ADH to LOS is 100 to 1, based on an estimated Mr of 3,000 for dLOS). Sulfo-NHS was added to a concentration of 8 mM, the pH was adjusted to 4.8, and EDC was added to a concentration of 0.1 M. The reaction mixture was stirred and maintained at pH 4.8 for 3 h. The reaction mixture was adjusted to pH 7.0 and passed through the G-50 column as described above. The eluate was assayed for carbohydrate and for ADH. The peaks containing both carbohydrate and AH were pooled, freeze-dried, and designated AH-dLOS. AH-dLOS was measured for its composition, using dLOS and ADH as standards |
| Antibodies | Polysera |
| Antibody Type Class | N/A |
| Assay System | Double immunodiffusion |
| Cross Reactivity | N/A |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera had complementmediated bactericidal activity against the homologous strain and heterologous strains of M. catarrhalis |
| Curator ID | AA + AS |
| Date of Curation | 24-09-2010 |
| References | 9573066 |
| PolysacDB ID | 1622 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Moraxella catarrhalis serotype C strain 26404 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to β-D-Galp-(1-->4)-α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-α-Kdop [branched to Kdop-(2-->4)] |
| BCSDB Structure | N/A |
| Proposed Function | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | 240 mg of lipooligosaccharide [LOS] was detoxified by using anhydrous hydrazine, and the dLOS was purified. Then adipic acid dihydrazide was conjugated to the dLOS (96 mg) in 12 ml of a 287 mM adipic acid dihydrazide suspension to form adipic hydrazide (AH)-dLOS derivatives, using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide HCl and N-hydroxysulfosuccinimide. The resulting AH-dLOS was finally coupled to Tetanus toxoid [TT]. 10 mg of TT (Mr, 150,000) was reacted with 20 mg of AH-dLOS (10 mg/ml) at a molar ratio of AH-dLOS to TT of 100:1 using 0.05 M 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide HCl. All reaction mixtures were maintained at pH 5.0 to 5.2 for 4 h at 4°C, and the reactions were stopped by adjusting the pH to 7.0. The reaction mixtures were dialyzed against 0.9% NaCl for 2 to 3 days, centrifuged, and passed through a Sephacryl S-300 column (2.6 by 90 cm) in 0.9% NaCl. Peaks that contained both protein and carbohydrate were pooled and designated dLOS-TT, dLOS-CRM-1, and dLOS-CRM-2. The three conjugates were analyzed to determine their carbohydrate and protein contents using dLOS and bovine serum albumin as standards |
| Antibodies | Polysera |
| Antibody Type Class | N/A |
| Assay System | ELISA, Bactericidal assay and bactericidal inhibition assay |
| Cross Reactivity | This antisera cross-reacted with Serotype A LPS and to a little extent to Serotype B |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera showed complement-mediated bactericidal activity against the homologous strain |
| Curator ID | AA + AS |
| Date of Curation | 24-09-2010 |
| References | PMC1932890 |
| PolysacDB ID | 1623 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Moraxella catarrhalis Serotype A strain 25238 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo |
| BCSDB Structure | N/A |
| Proposed Function | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Cross-reactive mutant (CRM9) of diphtheria toxin |
| Conjugate Method | Adipic acid dihydrazide (ADH) was bound to detoxified lipooligosaccharide [LOS] to form adipic hydrazide (AH)-dLOS derivatives, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) and N-hydroxysulfosuccinimide (sulfo-NHS). dLOS (70 mg) was dissolved in 7 ml of 345 mM ADH (molar ratio of ADH to LOS is 100 to 1, based on an estimated Mr of 3,000 for dLOS). Sulfo-NHS was added to a concentration of 8 mM, the pH was adjusted to 4.8, and EDC was added to a concentration of 0.1 M. The reaction mixture was stirred and maintained at pH 4.8 for 3 h. The reaction mixture was adjusted to pH 7.0 and passed through the G-50 column as described above. The eluate was assayed for carbohydrate and for AH. The peaks containing both carbohydrate and AH were pooled, freeze-dried, and designated AH-dLOS. AH-dLOS was measured for its composition, using dLOS and ADH as standards |
| Antibodies | Polysera |
| Antibody Type Class | N/A |
| Assay System | Enzyme-linked immunospot assay, ELISA and Bacterial challenge assay |
| Cross Reactivity | N/A |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | The dLOS-CRM vaccine induced a significant bacterial clearance (70 to 90%) of both homologous and heterologous strains in the lungs compared to that observed in the controls |
| Curator ID | AA + AS |
| Date of Curation | 25-09-2010 |
| References | PMC130355 |
| PolysacDB ID | 1624 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Moraxella catarrhalis serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo |
| BCSDB Structure | 22728 |
| Proposed Function | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature | Heat inactivated Moraxella catarrhalis serotype A |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 219A9 |
| Antibody Type Class | IgM |
| Assay System | ELISA, bactericidal assays and immunofluorescence assay |
| Cross Reactivity | MAb 219A9 was specific for a common epitope of A, B, and C M. catarrhalis serotypes. It also cross-reacted with whole bacteria and LPS antigens isolated from non-typeable H. influenzae and H. parainfluenzae strains |
| Proposed Epitope | 6-linked branch α-D-Galpara-(1-->4)-β-D-Galpara-(1-->4)-α-D-Glcpara-(1-->2)-β-D-lcpara seemed to be a part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab possessed a strong bactericidal effect against the three serotypes in the presence of complement |
| Curator ID | AA + AS |
| Date of Curation | 25-09-2010 |
| References | 17211546 |
| PolysacDB ID | 1625 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Moraxella catarrhalis serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo |
| BCSDB Structure | 22728 |
| Proposed Function | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature | Heat inactivated Moraxella catarrhalis serotype A |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 218A8 |
| Antibody Type Class | IgG3 |
| Assay System | ELISA, bactericidal assays and immunofluorescence assay |
| Cross Reactivity | Mab 218A8 reacted with two serotypes A and C |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for the detection of M. catarrhalis and its LOS antigen in clinical samples |
| Curator ID | AA + AS |
| Date of Curation | 25-09-2010 |
| References | 17211546 |
| PolysacDB ID | 1626 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Moraxella catarrhalis serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo |
| BCSDB Structure | 22728 |
| Proposed Function | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature | Heat inactivated Moraxella catarrhalis serotype A |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 217H4 |
| Antibody Type Class | IgG3 |
| Assay System | ELISA, bactericidal assays and immunofluorescence assay |
| Cross Reactivity | This Mab was specific to serotype A |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for the detection of M. catarrhalis and its LOS antigen in clinical samples |
| Curator ID | AA + AS |
| Date of Curation | 25-09-2010 |
| References | 17211546 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |