PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
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PolysacDB ID1797
Carbohydrate NameN-propionylated α(2-->8)-linked sialooligosaccharides   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameNeisseria meningitidis   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN-propionylated α(2-->8)-linked sialooligosaccharides
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureGlycoconjugates
Carrier NameTetanus toxoid
Conjugate MethodThe oligosaccharide was activated using NaIO4 at 4°C for 60 min and combined with monomeric tetanus toxoid in sodium phosphate buffer containing NaBH3CN and heated at 37°C for 5 days
AntibodiesMab 10B9
Antibody Type ClassIgG1
Assay SystemELISA, bactericidal assays
Cross ReactivityN/A
Proposed EpitopeThe epitope was located on short internal fragments of the polysaccharide
IEDB EpitopeN/A
Proposed UtilityThis Mab would help in the elucidation of the capsular polysaccharide structure
Curator IDAA + AS
Date of Curation03-11-2010
ReferencesPMC2196336

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PolysacDB ID1798
Carbohydrate NameN-propionylated α(2-->8)-linked sialooligosaccharides   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameNeisseria meningitidis   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN-propionylated α(2-->8)-linked sialooligosaccharides
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureGlycoconjugates
Carrier NameTetanus toxoid
Conjugate MethodThe oligosaccharide was activated using NaIO4 at 4°C for 60 min and combined with monomeric tetanus toxoid in sodium phosphate buffer containing NaBH3CN and heated at 37°C for 5 days
AntibodiesMab 7C11
Antibody Type ClassIgG1
Assay SystemELISA, bactericidal assays
Cross ReactivityN/A
Proposed EpitopeThe epitope was located on short internal fragments of the polysaccharide
IEDB EpitopeN/A
Proposed UtilityThis Mab would help in the elucidation of the capsular polysaccharide structure
Curator IDAA + AS
Date of Curation03-11-2010
ReferencesPMC2196336

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PolysacDB ID1799
Carbohydrate NameN-propionylated α(2-->8)-linked sialooligosaccharides   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameNeisseria meningitidis   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN-propionylated α(2-->8)-linked sialooligosaccharides
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureGlycoconjugates
Carrier NameTetanus toxoid
Conjugate MethodThe oligosaccharide was activated using NaIO4 at 4°C for 60 min and combined with monomeric tetanus toxoid in sodium phosphate buffer containing NaBH3CN and heated at 37°C for 5 days
AntibodiesMab 11G1
Antibody Type ClassIgG2a
Assay SystemELISA, bactericidal assays
Cross ReactivityN/A
Proposed EpitopeThe epitope was located on short internal fragments of the polysaccharide
IEDB EpitopeN/A
Proposed UtilityThis Mab would help in the elucidation of the capsular polysaccharide structure
Curator IDAA + AS
Date of Curation03-11-2010
ReferencesPMC2196336

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PolysacDB ID1800
Carbohydrate NameN-propionylated α(2-->8)-linked sialooligosaccharides   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameNeisseria meningitidis   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN-propionylated α(2-->8)-linked sialooligosaccharides
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureGlycoconjugates
Carrier NameTetanus toxoid
Conjugate MethodThe oligosaccharide was activated using NaIO4 at 4°C for 60 min and combined with monomeric tetanus toxoid in sodium phosphate buffer containing NaBH3CN and heated at 37°C for 5 days
AntibodiesMab 1F1
Antibody Type ClassIgG2b
Assay SystemELISA, bactericidal assays
Cross ReactivityN/A
Proposed EpitopeThe epitope was located on short internal fragments of the polysaccharide
IEDB EpitopeN/A
Proposed UtilityThis Mab would help in the elucidation of the capsular polysaccharide structure
Curator IDAA + AS
Date of Curation04-11-2010
ReferencesPMC2196336

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PolysacDB ID1801
Carbohydrate NameN-propionylated α(2-->8)-linked sialooligosaccharides   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameNeisseria meningitidis   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN-propionylated α(2-->8)-linked sialooligosaccharides
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureGlycoconjugates
Carrier NameTetanus toxoid
Conjugate MethodThe oligosaccharide was activated using NaIO4 at 4°C for 60 min and combined with monomeric tetanus toxoid in sodium phosphate buffer containing NaBH3CN and heated at 37°C for 5 days
AntibodiesMab 11F3
Antibody Type ClassIgG3
Assay SystemELISA, bactericidal assays
Cross ReactivityN/A
Proposed EpitopeThe epitope was located on short internal fragments of the polysaccharide
IEDB EpitopeN/A
Proposed UtilityThis Mab would help in the elucidation of the capsular polysaccharide structure
Curator IDAA + AS
Date of Curation04-11-2010
ReferencesPMC2196336

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