PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
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PolysacDB ID2497
Carbohydrate NameO-polysaccharide [Terminal trisaccharide]   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameVibrio cholerae O1 LPS serotype Inaba   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure6642
Proposed FunctionV. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodThe purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates
AntibodiesAntisera
Antibody Type ClassIgM and IgG2
Assay SystemELISA
Cross ReactivityThis antisera cross-reacted with V. cholerae Ogawa and Inaba LPS
Proposed EpitopeN/A
IEDB Epitope76775
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation05-06-2011
ReferencesPMC427411

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036