| PolysacDB ID | 2467 |
| Carbohydrate Name | Pneumococcal vaccine OP (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Streptococcus pneumoniae (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | This vaccine includes oligosaccharides of 6B, 14, 19F, 18C, 23F serotypes
|
| BCSDB Structure | N/A |
| Proposed Function | N/A |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxin mutant (CRM197) |
| Conjugate Method | The oligosaccharide-CRM197 conjugates were synthesized by either first hydrolysing the native oligosaccharide [PS] to smaller fragments and then subjecting the fragments to limited periodate oxidation or by oxidizing the native PS and then hydrolyzing it into smaller fragments. The PS-CRM197 conjugates were synthesized by oxidizing native pneumococcal PS with sodium metaperiodate to introduce random aldehyde functional groups. Oxidized OS or PS fragments were coupled to CRM197 in the presence of sodium cyanoborohydride |
| Antibodies | Human Antisera |
| Antibody Type Class | N/A |
| Assay System | ELISA, Opsonophagocytic killing assay |
| Cross Reactivity | The antisera reacted with 6B serotype, showed poor opsonophagocytic activity for 19F serotype. This antisera also cross-reacted with 6A serotype but did not react with 19A serotype |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | The findings suggest that some conjugate vaccines may elicit cross-protection against related polysaccharides better than others |
| Curator ID | AA + AS |
| Date of Curation | 26-05-2011
|
| References | 10515817 |