PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

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Record - 1 of 9 [TOP]

PolysacDB ID	1899
Carbohydrate Name	Unknown glycoprotein (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Pasteurella multocida serotype D (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Outer membrane proteins
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab MT2
Antibody Type Class	IgG2b
Assay System	ELISA, immunoelectron microscopy and immunoblotting
Cross Reactivity	This Mab was specific to serotype D and did not react with serotypes A, B, E, and F or field isolates of capsular serotype A of P. multocida
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	The Mab MT2 may be used effectively to subclassify serotype D strains
Curator ID	AA + AS
Date of Curation	24-11-2010
References	PMC228074

Record - 2 of 9 [TOP]

PolysacDB ID	1946
Carbohydrate Name	Unknown glycoprotein (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Blastocystis hominis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 6B6
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting and immunofluorescence
Cross Reactivity	This MAb exhibited some cross-reactivity with 2 reptilian Blastocystis isolates but not with Giardia intestinalis, Trichomonas vaginalis or Entamoeba histolytica.
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	03-12-2010
References	8773525

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PolysacDB ID	1947
Carbohydrate Name	Unknown glycoprotein (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Blastocystis hominis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 1E7
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting and immunofluorescence
Cross Reactivity	This MAb exhibited some cross-reactivity with 2 reptilian Blastocystis isolates but not with Giardia intestinalis, Trichomonas vaginalis or Entamoeba histolytica.
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	03-12-2010
References	8773525

Record - 4 of 9 [TOP]

PolysacDB ID	1948
Carbohydrate Name	Unknown glycoprotein (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Blastocystis hominis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 4f7
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting and immunofluorescence
Cross Reactivity	This MAb exhibited some cross-reactivity with 2 reptilian Blastocystis isolates but not with Giardia intestinalis, Trichomonas vaginalis or Entamoeba histolytica.
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	03-12-2010
References	8773525

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PolysacDB ID	1949
Carbohydrate Name	Unknown glycoprotein (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Blastocystis hominis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 4G11
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting and immunofluorescence
Cross Reactivity	This MAb exhibited some cross-reactivity with 2 reptilian Blastocystis isolates but not with Giardia intestinalis, Trichomonas vaginalis or Entamoeba histolytica.
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	04-12-2010
References	8773525

Record - 6 of 9 [TOP]

PolysacDB ID	2598
Carbohydrate Name	Unknown glycoprotein (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Rhizobium leguminosarum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Peribacteroid material
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab AFRC MAC 63
Antibody Type Class	N/A
Assay System	Immunoelectron microscopy
Cross Reactivity	This Mab cross-reacts with the peribacteroid membrane from Pisum sativum, plant plasma membranes and the Golgi bodies, but not the endoplasmic reticulum. This Mab also reacted with peribacteroid membranes from nodules of Vicia and lupin, and with plasma membranes and Golgi membranes from uninfected plant cells, including root tip cells from onion (Allium cepa), indicating that the antigen is highly conserved in the plasma membranes of plant cells
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab should prove useful in characterising the maturation and exchange of material between the Golgi apparatus and the plasma membrane of plant cells in general, quite apart from any specific applications as an immunochemical marker for the peribacteroid membrane in further studies on the Rhizobium-legume interaction
Curator ID	AA + AS
Date of Curation	14-07-2011
References	PMC554232

Record - 7 of 9 [TOP]

PolysacDB ID	2599
Carbohydrate Name	Unknown glycoprotein (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Rhizobium leguminosarum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Peribacteroid material
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab AFRC MAC 64
Antibody Type Class	N/A
Assay System	Immunoelectron microscopy
Cross Reactivity	This Mab cross-reacts with the peribacteroid membrane from Pisum sativum, plant plasma membranes and the Golgi bodies, but not the endoplasmic reticulum. This Mab also reacted with peribacteroid membranes from nodules of Vicia and lupin, and with plasma membranes and Golgi membranes from uninfected plant cells, including root tip cells from onion (Allium cepa), indicating that the antigen is highly conserved in the plasma membranes of plant cells
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab should prove useful in characterising the maturation and exchange of material between the Golgi apparatus and the plasma membrane of plant cells in general, quite apart from any specific applications as an immunochemical marker for the peribacteroid membrane in further studies on the Rhizobium-legume interaction
Curator ID	AA + AS
Date of Curation	15-07-2011
References	PMC554232

Record - 8 of 9 [TOP]

PolysacDB ID	2600
Carbohydrate Name	Unknown glycoprotein (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Rhizobium leguminosarum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Peribacteroid material
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab AFRC MAC 65
Antibody Type Class	N/A
Assay System	Immunoelectron microscopy
Cross Reactivity	This Mab cross-reacts with the peribacteroid membrane from Pisum sativum, plant plasma membranes and the Golgi bodies, but not the endoplasmic reticulum. This Mab also reacted with peribacteroid membranes from nodules of Vicia and lupin, and with plasma membranes and Golgi membranes from uninfected plant cells, including root tip cells from onion (Allium cepa), indicating that the antigen is highly conserved in the plasma membranes of plant cells
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab should prove useful in characterising the maturation and exchange of material between the Golgi apparatus and the plasma membrane of plant cells in general, quite apart from any specific applications as an immunochemical marker for the peribacteroid membrane in further studies on the Rhizobium-legume interaction
Curator ID	AA + AS
Date of Curation	15-07-2011
References	PMC554232

Record - 9 of 9 [TOP]

PolysacDB ID	2643
Carbohydrate Name	Unknown glycoprotein (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Cryptococcus neoformans serotype A [strain CSF-1] (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin killed whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab CSFi
Antibody Type Class	IgG
Assay System	ELISA, immunofluorescence, Western blotting, Adherence assays
Cross Reactivity	This antibody cross reacted with the outer surface of Cryptococcus neoformans serotype A [strain CSF] and a uncapsulated strain 52817
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab unlike other Mabs to Cryptococcus neoformans did not react with glucurunoxylomannan and hence was not specific to the capsular polysaccharide
Curator ID	AA + AS
Date of Curation	30-07-2011
References	15271943

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036