Details of SAPdb entry with Sequence E-α,β-ΔPhe |
Primary information | |
---|---|
SAPdb ID | 1491, |
PMID | 22833945 |
Peptide Name | Glu–alpha, beta-dehydrophenylalanine |
Peptide sequence | E-α,β-ΔPhe |
N-Terminal Modification | Free |
C-Terminal Modification | Free |
Non-Terminal Modification | α,β-ΔPhe=α,β-dehydrophenylalanine |
Length | 2 |
Peptide/Conjuagate | Peptide derivative |
Technique | TEM (Transmission Electron Microscopy) and DLS (Dynamic Light scattering) |
Solvent | 1,1,1,3,3,3-hexa-fluoro-isopropanol/water. |
Method | Stock solutions of dipeptides were prepared by dissolving 0.1–1 mg of them in 50 μl of HFIP. Solution heated and sonicated to dissolve the dipeptide. Assembly was initiated by addition of double-distilled water (1 ml) to the stock solution. Samples were aged 24–48 h before experiments. |
Conc | 0.1 - 1mg/ml |
Temperature | Room temperature |
Incubation Time | 24 -48 hours for sample aging and 30 - 60 minutes for self assembly |
Year | 2010 |
Self assembly | Yes |
Type of Self assembly | Vesicle |
Tertiary Structure (Technique) | Not Predicted), |
Linear | |
NA | |
Proteolytically stable | |
Nanovesicle | |
110 | |
EF | |
N.A. | |