Primary information |
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SAPdb_ID | 1489 |
PMID | 22788380 |
Year | 2012 |
Name | L-Carnosine |
Sequence | βAH |
N-Terminal Modification | Palmitic acid |
C-Terminal Modification | Free |
Non-terminal Modication | βA=beta-alanine |
Length | 2 |
Peptide/Conjugate/Mixture | Mixture |
Conjugate Partner | DPPC |
Technique | Cryo - TEM (Transmission Electron Microscopy) |
Solvent | Water |
Method | A weighted amount of DPPC+C16-βAH was dissolved in ethanol in a 100 mL round-bottom flask. The ethanol was removed to form a thin DPPC film on the walls of the flask under reduced pressure using a rotary evaporator. Any residual solvent was removed under a stream of N2. A weighed amount of water was then added. The flask was then returned to the rotoevaporator, immersed in a water bath, and vigorously rotated for ∼5 min, to reconstitute the lipid film at 50 °C. The solution was thenput in a flask and vortexed at 50 °C for an additional 5 min |
Concentration | 1 %wt |
pH | 6.5 - 1.5 |
Temperature
| 50°C |
Incubation Period | 5 - 10 minutes |
Self-Assembly Formation | Yes |
Type of Self-Assembly | Vesicles |
Size of Self-Assembled structure | ∼0.07 to 7.2 μm |
Linear/Cyclic | Linear |
Stability of Nanostructure | NA |
Comment | NA |
Secondary information |
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Physico-Chemical properties | |
STRUCTURE | |
SMILES | N.A. |