| Primary information |
|---|
| SAPdb_ID | 1541 |
| PMID | 21107375 |
| Year | 2010 |
| Name | Dipeptide 3 |
| Sequence | YL |
| N-Terminal Modification | Fmoc(fluorenylmethoxycarbonyl) |
| C-Terminal Modification | Free |
| Non-terminal Modication | None |
| Length | 2 |
| Peptide/Conjugate/Mixture | Peptide |
| Conjugate Partner | None |
| Technique | AFM (Atomic Force Microscopy) and DLS (Dynamic Light Scattering) |
| Solvent | Sodium phosphate buffer (pH 8) |
| Method | Peptide was dissolved in a 1 ml volume of 100 mM sodium phosphate buffer (pH 8) in the presence of varying concentrations of subtilisin (1.25–60.0 ml). The mixture was vortexed (30 s) and sonicated on ice for 20 min , and the low temperature ensures that no enzymatic conversion occurs up to this point. This was followed by heating,ceither in an oil bath or within the spectrophotometer using a temperature-controlled cuvet, at 55° for 60 min to allow enzymatic conversion to occur. The self-assembling system was then allowed to cool to room temperature. The gel samples were then left to stand for periods of 72 h. |
| Concentration | 10 mmol/kg |
| pH | 8 |
Temperature
| Intial 55°C heating for 1 hour and followed by cooling at room temperature |
| Incubation Period | 72 hour |
| Self-Assembly Formation | Yes |
| Type of Self-Assembly | Transparent gel |
| Size of Self-Assembled structure | NA |
| Linear/Cyclic | Linear |
| Stability of Nanostructure | Stable |
| Comment | NA |
| Secondary information |
|---|
| Physico-Chemical properties | |
| STRUCTURE | |
| SMILES | N.A. |
