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Details of TopicalPdb ID 1108

PRIMARY INFORMATION
ID	1108
PMID	20189781
Year	2010
Sequence	RALARALARALRALAR
Name	RALA
Length	16
N-Terminal Modification	Free
C-Terminal Modification	Free
Linear/ Cyclic	Linear
Chirality	L
Chemical Modification	None
Origin of Peptide	Synthetic,cell penetration enhancer
Nature of Peptide/Cargo	Based on a family of amphipatic peptides that exhibit improved membrane permeability belongs to a synthetic family of CPEs.
Mechanism	The penetration mechanism of these peptides into the cell and into the nucleus was proposed to be based on peptide aggregation within the bilayer surface.
Cargo Sequence/Structure	C14H10Cl2NNaO2, Sodium [o-(2,6-dichloroanilino)phenyl]acetate
Name of cargo	Sodium diclofenac, Hexagonal lypotropic liquid crystals (HIILC)
Assay	Franz diffusion cell, HPLC
Enhancer	73.8 wt% GMO and 8.2 wt% TAG (9:1 weight ratio) at 18 wt% water content. The hexagonal liquid crystals (HIILC) were prepared by mixing weighed quantities of GMO and TAG while heating to 45 ◦C. This was done in sealed tubes under nitrogen atmosphere to avoid oxidation of the GMO. An appropriate quantity of preheated water at the same temperature was added, and the samples were stirred and cooled to 25 ◦C. RALA was solubilized up to its maximal capacity in each system—in the range of 1–6 wt%
Properties of enhancer	Enhances permeability
Concentration	1.4 wt%
Incubation time	25 hours
Tissue permeability (value with units)	60% of applied dose
Tissue Sample	Stratum corneum of ear of porcine
Ex vivo/In vivo/In vitro	ex vivo
SECONDARY INFORMATION
STRUCTURE	Predicted Structure
SMILES	N[C@@H](CCCNC(=[NH2])N)C(=O)N[C@@H] (C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O) N[C@@H](CCCNC(=[NH2])N)C(=O)N[C@@H](C)C(=O)N[C@@H] (CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC (=[NH2])N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C) C)C(=O)N[C@@H](CCCNC(=[NH2])N)C(=O)N [C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H] (C)C(=O)N[C@@H](CCCNC(=[NH2])N)C=O