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Details of TopicalPdb ID 1364

PRIMARY INFORMATION
ID1364
PMID20891012
Year2010
SequenceGRKKRRQRRRPPQRKC
MSKGEELFTGVVPILV
ELDGDVNGHKFSVSGE
GEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTF
SYGVQCFSRYPDHMKQ
HDFFKSAMPEGYVQE
RTIFFKDDGNYKTRAE
VKFEGDTLVNRIELKG
IDFKEDGNILGHKLEY
NYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGS
VQLADHYQQNTPIGDG
PVLLPDNHYLSTQSAL
SKDPNEKRDHMVLLEF
NameN-terminal Tat fusion green fluorescent protein (GFP) (TG)
Length254
N-Terminal ModificationFree
C-Terminal ModificationFree
Linear/ CyclicLinear
ChiralityL
Chemical ModificationNone
Origin of PeptideSynthetic fusion protein of TAT and GFP
Nature of Peptide/CargoFluorescent CPP
MechanismNot mentioned
Cargo Sequence/StructureNone
Name of cargo
Not applicable
AssayVertical Franz diffusion cell
EnhancerGFP solution in 100 mM phosphate buffer (pH 7.0)
Properties of enhancerNot mentioned
Concentration10µM
Incubation time6 hours
Tissue permeability (value with units)Cumulative amounts 4.86 ± 0.55 µg/cm2 and fluxes 0.81 ± 0.09 µg/cm2·h for stratum corneum. The receiver compartment of Franz Diffusion Cells had cumulative amount=28.50 ± 12.07 µg/cm2 and fluxes= 4.75 ± 1.47 µg/cm2·h
Tissue SampleStratum corneum of the abdominal skin of Sparague-Dawley rats after shaving off hair
Ex vivo/In vivo/In vitroex vivo
SECONDARY INFORMATION
STRUCTURE
Predicted Structure
SMILESN.A.