Welcome to Entry Card of Peptide


Details of TopicalPdb ID 1441

PRIMARY INFORMATION
ID1441
PMID3262039
Year1992
SequenceACDTATCVTHRLAGL
LSRSGGVVKNNFVPT
NVGSKAF
Nameα-CGRP (α-calcitonin gene-related peptide)
Length37
N-Terminal ModificationFree
C-Terminal ModificationFree
Linear/ CyclicLinear
ChiralityL
Chemical ModificationNone
Origin of PeptideCGRP is encoded in the same gene as calcitonin and expressed in neural tissues by processing through a different mRNA pathway
Nature of Peptide/CargoSpecific binding sites for CGRP have been demonstrated both in the brain and in peripheral target tissues, where CGRP has been shown to act through a CAMP-dependent mechanism
MechanismCGRP is encoded in the same gene as calcitonin and expressed in neural tissues by processing through a different mRNA pathway
Cargo Sequence/StructureNone
Name of cargo
Not applicable
AssayLowry method for protein estimation, cAMP content via 125I-radioimmunoassay, Electromanometrical recording intraocular pressure
EnhancerNot mentioned
Properties of enhancerNot mentioned
ConcentrationCGRP=2.0µg, Methysergide=1mg/Kg(i.v.)
Incubation time30 minutes
Tissue permeability (value with units)Protein=13.3±6.2mg/ml, cAMP=147.0±7.4pmol/ml, Intraocular pressure=19mmHg at 5 min, 21mmHg at 10 min, 26mmHg at 15 min, 31mmHg at 28 min, 35.5mmHg at 30 minutes.
Tissue SampleEyes of New Zealand albino rabbits
Ex vivo/In vivo/In vitroin vivo
SECONDARY INFORMATION
STRUCTURE
Predicted Structure
SMILESN[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N
[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H]
(C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N
[C@H](C(=O)NCC(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H]
(C(=O)N[C@H](C(=O)N[C@H](C(=O)NCC(=O)NCC
(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H]
(C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C
(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)NCC
(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N
[C@H](C=O)Cc1ccccc1)C)CCCCN)CO)C(C)C)CC(=O)N)[C@H](O)
C)CCC)C(C)C)Cc1ccccc1)CC(=O)N)CC(=O)N)CCCCN)
C(C)C)C(C)C)CO)CCCNC(=N)N)CO)CC(C)C)CC(C)C)C)
CC(C)C)CCCNC(=N)N)Cc1[nH]cnc1)[C@H]
(O)C)C(C)C)CS)[C@H](O)C)C)[C@H](O)C)CC(=O)O)CS)C