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Details of TopicalPdb ID 1540

PRIMARY INFORMATION
ID1540
PMID22386653
Year2012
SequenceQNPASPPEEGSPDPDSTGA
LVEEEDPFFKVPVNKLA
AAVSNFGYDLYRVRSSTS
PTTNVLLSPLSVATAL
SALSLGAEQRTESIIHR
ALYYDLISSPDIHGTYKEL
LDTVTAPQKNLKSAS
RIVFEKKLRIKSSFVAPLE
KSYGTRPRVLTGNP
RLDLQEINNWVQAQ
MKGKLARSTKEIPDEIS
ILLLGVAHFKGQWVTKF
DSRKTSLEDFYLDEER
TVRVPMMSDPKAVLR
YGLDSD
NamePEDF (Pigment epithelium-derived factor)
Length399
N-Terminal ModificationPyrrolidone carboxylic acid
C-Terminal ModificationFree
Linear/ CyclicLinear
ChiralityL
Chemical ModificationGlycosylation at 266th residue, phosphoserine at 5, 95 and 108th residue
Origin of PeptideNeurotrophic protein
Nature of Peptide/CargoIt induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis.
MechanismNeurotrophic protein
Cargo Sequence/StructureNone
Name of cargo
Not applicable
AssayVEGF and 8-OHdG Immunostaining, Measurement of corneal neo-vascularization via avidin-biotin-alkaline phosphatase kit and statistical analysis
EnhancerNone
Properties of enhancerNot applicable
Concentration1 eye drop of 1 µM thrice a day for 4 days
Incubation time4 Days
Tissue permeability (value with units)Neo-vascularized area= 18% due to PEDF (PBS=20%)
Tissue SampleSeven week-old male Spraque–Dawley rat's right eye was used as test and left eye considered as control (PBS)
Ex vivo/In vivo/In vitroin vivo
SECONDARY INFORMATION
STRUCTURE
Predicted Structure
SMILESN.A.