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siRNA Id: | aspsirna0902
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siRNA Name: | siAhc04
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| Gene: | MHC, class I, A (HLA-A) heavy chain |
| Disease: | HLA incompatibility |
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Wild allele/target (5'->3'): | AGAUACACCUGCCAUGUGC
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Mutant allele/target (5'->3'): | AGAUACUCCUGCCAUGUGC
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Target Mutation: | c.*66A>T
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Wild siRNA (As strand): | UCUAUGUGGACGGUACACG
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ASP-siRNA (As strand): | UCUAUGAGGACGGUACACG
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Mismatch position in siRNA: | A13U |
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Mutant allele (5'->3'): | AGAUACUCCUGCCAUGUGC
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ASP-siRNA (As strand): | UCUAUGAGGACGGUACACG
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ASP-siRNA efficacy for mutant allele: | 70
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Wild allele (5'->3'): | AGAUACACCUGCCAUGUGC
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ASP-siRNA (As strand): | UCUAUGAGGACGGUACACG
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ASP-siRNA efficacy for wild allele: | NA
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Percentage discrimination for ASP-siRNA: | NA
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Wild allele (5'->3'): | AGAUACACCUGCCAUGUGC
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Wild siRNA (As strand): | UCUAUGUGGACGGUACACG
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Wild sirna efficacy for wild allele: | 50
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Wild allele (5'->3'): | AGAUACACCUGCCAUGUGC
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ASP-siRNA (As strand): | UCUAUGAGGACGGUACACG
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Wild sirna efficacy for mutant allele: | 90
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Percentage discrimination for wild siRNA: | -40
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| Concentration: | 1.2mIcrog |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P01892 |
| Target sequence: | CGGTTCGACAGCGACGCCGCG |
| Genbank Accesion: | NM_001242758.1 |
| Cell-line: | Epstein–Barr-virus-immortalized B-lymphocytes |
| Experimental technique used: | Western blot |
| Transfection method: | RNAiFect |
| siRNA expression method: | Ambion |
| Post-transfection duration: | 48 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 16520945 |
