BioPhytMol: A database of Phytomolecules against Mycobacteria

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Page - 1 of 1 Record - 1 of 8 [TOP]

Compound ID

3670

Compound Structure

Plant Source

Micromelum hirsutum Common Name:

Source Family

Rutaceae

Origin

Plant Part Used

Stem bark

Extract

Dichloromethane

Target Bacteria

Mycobacterium tuberculosis H37Rv

Assay / Test Done

Fluorometric Alamar Blue Microassay (FMABA)

Positive Control Used (conc.)

Rifampin (0.040 ± 0.017 µg/ml)

Inhibition [%]

> 90 %

Activity [MIC] µg/ml

1.5 ± 0.4 µg/ml

Activity (In terms of dilution)

Activity (Zone of inhibition in mm)

Active Compound Identified

(-) - Z - 9 - Octadecene - 4 - Olide [Micromolide]

PubChem ID

Ethnomedicinal Information

PubMed ID [Source Literature]

15770548

Extract Preparation

The dried, milled stem bark of Micromelum hirsutum was extracted with CH₂Cl₂ to yield 45.5 g extract, of which 32.1 g were chromatographed by vacuum liquid chromatography over a silica gel column

Chemical Classification [Active Compound]

Alicyclic, Butenolide, Lactone, Alkene

Media / Broth Used [Antimicrobial Assay/Test]

Cytotoxicity Assay [AID]

Against Vero cells (ATCCCCL- 81) in the CellTiter 96 aqueous nonradioactive cell proliferation assay

Molecular Weight

280.240

Molecular Formula

C₁₈H₃₂O₂

SMILES

O1C(=O)CCC1CCCC/C=CCCCCCCCC

XLogP

7.349

PSA

26.300

H-bond Donor

H-bond Acceptor

No. of Rotatable Bond Count

No. of Rings

No. of N

No. of O

No. of S

Reference(s)

1) Ma C, Case RJ, Wang Y, Zhang HJ, Tan GT, Van Hung N, Cuong NM, Franzblau SG, Soejarto DD, Fong HH, Pauli GF.Anti-tuberculosis constituents from the stem bark of Micromelum hirsutum.Planta Med. 2005 Mar;71(3):261-7

Curator

Vikramjitmandal

Record - 2 of 8 [TOP]

Compound ID

3671

Compound Structure

Plant Source

Micromelum hirsutum Common Name:

Source Family

Rutaceae

Origin

Plant Part Used

Stem bark

Extract

Dichloromethane

Target Bacteria

Mycobacterium tuberculosis H37Rv

Assay / Test Done

Fluorometric Alamar Blue Microassay (FMABA)

Positive Control Used (conc.)

Rifampin (0.040 ± 0.017 µg/ml)

Inhibition [%]

> 90 %

Activity [MIC] µg/ml

31.5 ± 0.2 µg/ml

Activity (In terms of dilution)

Activity (Zone of inhibition in mm)

Active Compound Identified

Micromeline

PubChem ID

5278450

Ethnomedicinal Information

PubMed ID [Source Literature]

15770548

Extract Preparation

The dried, milled stem bark of Micromelum hirsutum was extracted with CH₂Cl₂ to yield 45.5 g extract, of which 32.1 g were chromatographed by vacuum liquid chromatography over a silica gel column

Chemical Classification [Active Compound]

Aromatic, Tricyclic, Alkaloid, Carbazole, Prenylated, Aldehyde, Phenol

Media / Broth Used [Antimicrobial Assay/Test]

Cytotoxicity Assay [AID]

Against Vero cells (ATCCCCL- 81) in the CellTiter 96 aqueous nonradioactive cell proliferation assay

Molecular Weight

279.126

Molecular Formula

C₁₈H₁₇NO₂

SMILES

Oc1c(c2c3c([nH]c2cc1)ccc(c3)C=O)CC=C(C)C

XLogP

3.814

PSA

53.090

H-bond Donor

H-bond Acceptor

No. of Rotatable Bond Count

No. of Rings

No. of N

No. of O

No. of S

Reference(s)

Curator

Vikramjitmandal

Record - 3 of 8 [TOP]

Compound ID

3672

Compound Structure

Plant Source

Micromelum hirsutum Common Name:

Source Family

Rutaceae

Origin

Plant Part Used

Stem bark

Extract

Dichloromethane

Target Bacteria

Mycobacterium tuberculosis H37Rv

Assay / Test Done

Fluorometric Alamar Blue Microassay (FMABA)

Positive Control Used (conc.)

Rifampin (0.040 ± 0.017 µg/ml)

Inhibition [%]

> 90 %

Activity [MIC] µg/ml

14.3 ± 0.9 µg/ml

Activity (In terms of dilution)

Activity (Zone of inhibition in mm)

Active Compound Identified

Lansine

PubChem ID

5317755

Ethnomedicinal Information

PubMed ID [Source Literature]

15770548

Extract Preparation

The dried, milled stem bark of Micromelum hirsutum was extracted with CH₂Cl₂ to yield 45.5 g extract, of which 32.1 g were chromatographed by vacuum liquid chromatography over a silica gel column

Chemical Classification [Active Compound]

Aromatic, Tricyclic, Alkaloid, Carbazole, Aldehyde, Ether

Media / Broth Used [Antimicrobial Assay/Test]

Cytotoxicity Assay [AID]

Against Vero cells (ATCCCCL- 81) in the CellTiter 96 aqueous nonradioactive cell proliferation assay

Molecular Weight

255.090

Molecular Formula

C₁₅H₁₃NO₃

SMILES

O(c1cc2[nH]c3c(c2cc1C=O)ccc(OC)c3)C

XLogP

2.419

PSA

51.320

H-bond Donor

H-bond Acceptor

No. of Rotatable Bond Count

No. of Rings

No. of N

No. of O

No. of S

Reference(s)

Curator

Vikramjitmandal

Record - 4 of 8 [TOP]

Compound ID

3673

Compound Structure

Plant Source

Micromelum hirsutum Common Name:

Source Family

Rutaceae

Origin

Plant Part Used

Stem bark

Extract

Dichloromethane

Target Bacteria

Mycobacterium tuberculosis H37Rv

Assay / Test Done

Fluorometric Alamar Blue Microassay (FMABA)

Positive Control Used (conc.)

Rifampin (0.040 ± 0.017 µg/ml)

Inhibition [%]

> 90 %

Activity [MIC] µg/ml

> 128 µg/ml

Activity (In terms of dilution)

Activity (Zone of inhibition in mm)

Active Compound Identified

3 - Methylcarbazole

PubChem ID

20746

Ethnomedicinal Information

PubMed ID [Source Literature]

15770548

Extract Preparation

The dried, milled stem bark of Micromelum hirsutum was extracted with CH₂Cl₂ to yield 45.5 g extract, of which 32.1 g were chromatographed by vacuum liquid chromatography over a silica gel column

Chemical Classification [Active Compound]

Aromatic, Tricyclic, Carbazole, Alkaloid

Media / Broth Used [Antimicrobial Assay/Test]

Cytotoxicity Assay [AID]

Against Vero cells (ATCCCCL- 81) in the CellTiter 96 aqueous nonradioactive cell proliferation assay

Molecular Weight

181.089

Molecular Formula

C₁₃H₁₁N

SMILES

[nH]1c2c(c3c1cccc3)cc(cc2)C

XLogP

4.401

PSA

15.790

H-bond Donor

H-bond Acceptor

No. of Rotatable Bond Count

No. of Rings

No. of N

No. of O

No. of S

Reference(s)

Curator

Vikramjitmandal

Record - 5 of 8 [TOP]

Compound ID

3674

Compound Structure

Plant Source

Micromelum hirsutum Common Name:

Source Family

Rutaceae

Origin

Plant Part Used

Stem bark

Extract

Dichloromethane

Target Bacteria

Mycobacterium tuberculosis H37Rv

Assay / Test Done

Fluorometric Alamar Blue Microassay (FMABA)

Positive Control Used (conc.)

Rifampin (0.040 ± 0.017 µg/ml)

Inhibition [%]

> 90 %

Activity [MIC] µg/ml

> 128 µg/ml

Activity (In terms of dilution)

Activity (Zone of inhibition in mm)

Active Compound Identified

Methyl Carbazole - 3 - Carboxylate

PubChem ID

504069

Ethnomedicinal Information

PubMed ID [Source Literature]

15770548

Extract Preparation

The dried, milled stem bark of Micromelum hirsutum was extracted with CH₂Cl₂ to yield 45.5 g extract, of which 32.1 g were chromatographed by vacuum liquid chromatography over a silica gel column

Chemical Classification [Active Compound]

Aromatic, Tricyclic, Alkaloid, Carbazole, Ester

Media / Broth Used [Antimicrobial Assay/Test]

Cytotoxicity Assay [AID]

Against Vero cells (ATCCCCL- 81) in the CellTiter 96 aqueous nonradioactive cell proliferation assay

Molecular Weight

225.079

Molecular Formula

C₁₄H₁₁NO₂

SMILES

O(C(=O)c1cc2c3c([nH]c2cc1)cccc3)C

XLogP

3.425

PSA

42.090

H-bond Donor

H-bond Acceptor

No. of Rotatable Bond Count

No. of Rings

No. of N

No. of O

No. of S

Reference(s)

Curator

Vikramjitmandal

Record - 6 of 8 [TOP]

Compound ID

3675

Compound Structure

Plant Source

Micromelum hirsutum Common Name:

Source Family

Rutaceae

Origin

Plant Part Used

Stem bark

Extract

Dichloromethane

Target Bacteria

Mycobacterium tuberculosis H37Rv

Assay / Test Done

Fluorometric Alamar Blue Microassay (FMABA)

Positive Control Used (conc.)

Rifampin (0.040 ± 0.017 µg/ml)

Inhibition [%]

> 90 %

Activity [MIC] µg/ml

42.3 ± 0.5 µg/ml

Activity (In terms of dilution)

Activity (Zone of inhibition in mm)

Active Compound Identified

3 - Formylcarbazole

PubChem ID

20746

Ethnomedicinal Information

PubMed ID [Source Literature]

15770548

Extract Preparation

The dried, milled stem bark of Micromelum hirsutum was extracted with CH₂Cl₂ to yield 45.5 g extract, of which 32.1 g were chromatographed by vacuum liquid chromatography over a silica gel column

Chemical Classification [Active Compound]

Aromatic, Tricyclic, Carbazole, Alkaloid

Media / Broth Used [Antimicrobial Assay/Test]

Cytotoxicity Assay [AID]

Against Vero cells (ATCCCCL- 81) in the CellTiter 96 aqueous nonradioactive cell proliferation assay

Molecular Weight

181.089

Molecular Formula

C₁₃H₁₁N

SMILES

[nH]1c2c(c3c1cccc3)cc(cc2)C

XLogP

4.401

PSA

15.790

H-bond Donor

H-bond Acceptor

No. of Rotatable Bond Count

No. of Rings

No. of N

No. of O

No. of S

Reference(s)

Curator

Vikramjitmandal

Record - 7 of 8 [TOP]

Compound ID

3676

Compound Structure

Plant Source

Micromelum hirsutum Common Name:

Source Family

Rutaceae

Origin

Plant Part Used

Stem bark

Extract

Dichloromethane

Target Bacteria

Mycobacterium tuberculosis H37Rv

Assay / Test Done

Fluorometric Alamar Blue Microassay (FMABA)

Positive Control Used (conc.)

Rifampin (0.040 ± 0.017 µg/ml)

Inhibition [%]

> 90 %

Activity [MIC] µg/ml

15.6 ± 0.2 µg/ml

Activity (In terms of dilution)

Activity (Zone of inhibition in mm)

Active Compound Identified

3 - Formyl - 6 - Methoxycarbazole

PubChem ID

5278452

Ethnomedicinal Information

PubMed ID [Source Literature]

15770548

Extract Preparation

The dried, milled stem bark of Micromelum hirsutum was extracted with CH₂Cl₂ to yield 45.5 g extract, of which 32.1 g were chromatographed by vacuum liquid chromatography over a silica gel column

Chemical Classification [Active Compound]

Aromatic, Tricyclic, Carbazole, Alkaloid, Aldehyde, Ether

Media / Broth Used [Antimicrobial Assay/Test]

Cytotoxicity Assay [AID]

Against Vero cells (ATCCCCL- 81) in the CellTiter 96 aqueous nonradioactive cell proliferation assay

Molecular Weight

225.079

Molecular Formula

C₁₄H₁₁NO₂

SMILES

O(c1cc2c3c([nH]c2cc1)ccc(c3)C=O)C

XLogP

2.863

PSA

42.090

H-bond Donor

H-bond Acceptor

No. of Rotatable Bond Count

No. of Rings

No. of N

No. of O

No. of S

Reference(s)

Curator

Vikramjitmandal

Record - 8 of 8 [TOP]

Compound ID

3677

Compound Structure

Plant Source

Micromelum hirsutum Common Name:

Source Family

Rutaceae

Origin

Plant Part Used

Stem bark

Extract

Dichloromethane

Target Bacteria

Mycobacterium tuberculosis Erdman (ATCC 35 801)

Assay / Test Done

Positive Control Used (conc.)

Rifampin

Inhibition [%]

> 90 %

Activity [MIC] µg/ml

Activity (In terms of dilution)

Activity (Zone of inhibition in mm)

Active Compound Identified

5α - Epoxyalantolactone

PubChem ID

474521

Ethnomedicinal Information

PubMed ID [Source Literature]

15770548

Extract Preparation

The dried, milled stem bark of Micromelum hirsutum was extracted with CH₂Cl₂ to yield 45.5 g extract, of which 32.1 g were chromatographed by vacuum liquid chromatography over a silica gel column

Chemical Classification [Active Compound]

Alicyclic, Tricyclic, Terpene, Sesquiterpene, Lactone, Epoxy

Media / Broth Used [Antimicrobial Assay/Test]

Cytotoxicity Assay [AID]

Against Vero cells (ATCCCCL- 81) in the CellTiter 96 aqueous nonradioactive cell proliferation assay

Molecular Weight

248.141

Molecular Formula

C₁₅H₂₀O₃

SMILES

O1[C@]23[C@@](C[C@H]4OC(=O)C(=C)[C@H]4[C@H]12)(CCC[C@@H]3C)C

XLogP

2.414

PSA

38.830

H-bond Donor

H-bond Acceptor

No. of Rotatable Bond Count

No. of Rings

No. of N

No. of O

No. of S

Reference(s)

Curator

Vikramjitmandal

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