| Compound ID | 3861 |
| Compound Structure |  |
| Plant Source | Rhus taitensis Guill. Common Name:Pumac |
| Source Family | Anacardiaceae |
| Origin | Papua New Guinea |
| Plant Part Used | Leaf, twig |
| Extract | Methanol |
| Target Bacteria | Mycobacterium tuberculosis H37Ra (ATCC 25177) |
| Assay / Test Done | MTT Assay |
| Positive Control Used (conc.) | Rifampin (0.0025 - 0.0079 µg/ml) |
| Inhibition [%] | NR |
| Activity [MIC] µg/ml | 10 µg/ml |
| Activity (In terms of dilution) | NR |
| Activity (Zone of inhibition in mm) | NR |
| Active Compound Identified | Tetrahydroxysqualene |
| PubChem ID | 25058109 |
| Ethnomedicinal Information | NR |
| PubMed ID [Source Literature] | 18710283 |
| Extract Preparation | Air - dried leaves and twigs (108.6 g) of Rhus taitensis were ground and extracted with 250 ml MeOH (3 × 24 hours) at room temperature. The crude extract (1.7 g) was filtered and concentrated in vacuo. 5 grams of the crude extract were dissolved in MeOH and mixed with 5 g of HP20SS and dried. The mixture was then poured into a column and fractionated using 100 % water, 75 % H2O / 25 % 2 - propanol, 50 % H2O / 50 % 2 - propanol, 25 % H2O / 75 % 2 - propanol, and 100 % MeOH to yield five fractions designated FW, F1, F2, F3, F4, respectively. The fractions were collected and the solvents evaporated using a centrifugal evaporator |
| Chemical Classification [Active Compound] | Aliphatic, Alkene, Terpene, Triterpene, Alcohol |
| Media / Broth Used [Antimicrobial Assay/Test] | ADC (Remel) supplemented 7H9 medium |
| Cytotoxicity Assay [AID] | Against Human T cells |
| Molecular Weight | 474.371 |
| Molecular Formula | C30H50O4 |
| SMILES | OCC(=CCC/C(=C/CC/C(=C/CC/C=C(/CC/C=C(/CCC=C(CO)CO)C)C)/C)/C)CO |
| XLogP | 5.646 |
| PSA | 80.920 |
| H-bond Donor | 4 |
| H-bond Acceptor | 4 |
| No. of Rotatable Bond Count | 19 |
| No. of Rings | 0 |
| No. of N | 0 |
| No. of O | 4 |
| No. of S | 0 |
| Reference(s) | 1) Noro JC, Barrows LR, Gideon OG, Ireland CM, Koch M, Matainaho T, Piskaut P, Pond CD, Bugni TS.Tetrahdroxysqualene from Rhus taitensis shows antimycobacterial activity against Mycobacterium tuberculosis.J Nat Prod. 2008 Sep;71(9):1623-4. Epub 2008 Aug 19
|
| Curator | KeyaMukherjee, Farzana Shamsudeen, gppreetha |
