CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr4645 details


CrisprIDCrispr4645
OrganismFundulus heteroclitus
Gene/Locus (Literature)AHR2b
NCBI Gene NameAHR2
Target SequenceCCAGACCGCTCAGACCTTCGCCCC
Target+Mutant_Flanking

CCGGCTCCAACGGCTCCAGCTGGACCGCCGAGCGGACCCCGGTGTTCGGAGGAAGCGTCCAGACCGCTCAGACCTTCGCCCCAGAGGCGACCTCCATCGACGGCGTCAGCTTCTCC
CCGGCTCCAACGGCTCCAGCTGGACCGCCGAGCGGACCCCGGTGTTCGGAGGAAGCGTCCAGACC - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - TCAGCTTCTCC

Modification LengthD40
Genetic ModificationDeletion
LocationExon3
Conc. (ng/μl) NA
Cell line Embryo
Method/AssaySurveyor assay/Sequencing
Crispr efficiency 63%
PRM/crRNA/VectorT7
PRM-cas/vectorT7
Pubmed25481785