Field | Sense | Antisense |
siRNA chemical modification | 0 | Phosphorothioate |
siRNA modification types | 0 | 1 |
Overall number of modifications | 0 | 3 |
Position of modifications | 0 | 12,13,14 |
Modification on sugar or base or phosphate | 0 | Phosphate-linkage |
SMILES (Click to view structure & nomenclature) |
- |
OCC1OCC(O)C1OP(O)(S)=O |
siRNA Sequence | AAGUAAGGACCAGAGACAAA | UUUGUCUCUGGUCCUUACUU |
siRNA length base-pair | 20 | 20 |
siRNA name in paper | 308746 | 336673 |
Biological activity | IC-50 = 0.122 nM for target mRNA | |
Experiment used to check activity | RT-PCR | |
Melting temperature (oC) | NA | |
Target gene | PTEN | |
siRNA concentration | 50 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | LIPOFECTIN | |
Duration after transfection | 16 Hours | |
Article title | Positionally modified siRNA constructs |
|
Reference | US 8394947 B2 |