Raghava, G.P.S. and Joshi, A and Agrewala, J N (1992) Calculation of antibody and antigen concentrations from ELISA data using a graphical method. Journal of immunological methods, 153 (1-2). pp. 263-4. ISSN 0022-1759
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Abstract
A graphical method for determining the concentration of either the antibody or the antigen from ELISA data is presented in the form of a GWBASIC program. In the program, ELISAEQ, optical densities (OD) obtained from a 96-well ELISA plate can be input either directly by interfacing a microplate reader to the computer or manually. The program uses standard sample data, and selects the semilogarithmic linear range. Over this range, a least-squares method is used to determine the concentrations of interest. In addition, a hyperbolic interpolation formula is derived over the entire range for estimating the antibody or antigen concentration of the unknown samples whose OD is beyond the linear range.
Item Type: | Article |
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Additional Information: | Copyright of this article belongs to Elsevier Science. |
Subjects: | Q Science > QR Microbiology > QR180 Immunology |
Depositing User: | Dr. K.P.S.Sengar |
Date Deposited: | 09 Jan 2012 07:16 |
Last Modified: | 28 Mar 2014 10:57 |
URI: | http://crdd.osdd.net/open/id/eprint/114 |
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