Calculation of antibody and antigen concentrations from ELISA data using a graphical method.

Raghava, G.P.S. and Joshi, A and Agrewala, J N (1992) Calculation of antibody and antigen concentrations from ELISA data using a graphical method. Journal of immunological methods, 153 (1-2). pp. 263-4. ISSN 0022-1759

PDF raghava1992.pdf - Published Version Restricted to Registered users only Download (94Kb) | Request a copy

Abstract

A graphical method for determining the concentration of either the antibody or the antigen from ELISA data is presented in the form of a GWBASIC program. In the program, ELISAEQ, optical densities (OD) obtained from a 96-well ELISA plate can be input either directly by interfacing a microplate reader to the computer or manually. The program uses standard sample data, and selects the semilogarithmic linear range. Over this range, a least-squares method is used to determine the concentrations of interest. In addition, a hyperbolic interpolation formula is derived over the entire range for estimating the antibody or antigen concentration of the unknown samples whose OD is beyond the linear range.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Subjects:	Q Science > QR Microbiology > QR180 Immunology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	09 Jan 2012 07:16
Last Modified:	28 Mar 2014 10:57
URI:	http://crdd.osdd.net/open/id/eprint/114

Actions (login required)

View Item