Pseudomonas Pichavaramensis sp. nov., Isolated from Brackish Water of Pichavaram Mangrove forest, Tamil Nadu

Mittal, Sumit and Kushwaha, Hariom and Nandini, N and Choksket, Stanzin and Prabhu, Devachandana C. and Pinnaka, Anil Kumar and Korpole, Suresh (2026) Pseudomonas Pichavaramensis sp. nov., Isolated from Brackish Water of Pichavaram Mangrove forest, Tamil Nadu. CURRENT MICROBIOLOGY, 83 (4).

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Abstract

A cream-white bacterial colony designated SH1-B was isolated on Tryptone Soya Agar (TSA) plated with a brackish water sample collected from the Pichavaram mangrove forest. The strain SH1-B was Gram-stain negative, rod-shaped, motile, non-spore-forming aerobic bacterium. Catalase and oxidase activities were positive. Cells grew between 15 and 37 degrees C and pH 5.0-9.0. The nucleotide sequence of 16 S rRNA gene displayed high similarity with species of the genus Pseudomonas, including P. khazarica TBZ2(T) (98.5%), P. yangonensis MY50(T) (98.0%) and P. oleovorans subsp. oleovorans DSM1045(T) (98.0%). Genome sequence analysis of strain SH1-B showed 74.2-87.6% average nucleotide identity (ANI) with the type strains of the closest species. Additionally, digital DNA-DNA hybridization (dDDH) analysis revealed low relatedness, with a maximum value of 34.9% observed for its closest phylogenetic relative, P. khazarica TBZ2(T). The G + C content of the genomic DNA was 62.2%. The major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and an unidentified phospholipid (PL1). The predominant cellular fatty acids of strain SH1-B included C-18:1 omega 7c, C-16:0,C- C-12:0,C- summed feature 3 (C-16:1 omega 6c/C-16:1 omega 7c). Considering the phenotypic differences and low genomic identity compared to the closest species, strain SH1-B was identified as a novel species within the genus Pseudomonas, for which the name Pseudomonas pichavaramensis sp. nov. is proposed. The type strain is SH1-B-T (= MTCC 13546(T) = JCM 37389(T) = ICMP 25819(T)).

Item Type:	Article
Additional Information:	copyright of this article belongs to SPRINGER
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	16 Jul 2026 03:56
Last Modified:	16 Jul 2026 03:56
URI:	http://crdd.osdd.net/open/id/eprint/3450

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