Silk-fiber immobilized lipase-catalyzed hydrolysis of emulsified sunflower oil.

Chatterjee, Sushovan and Barbora, Lepakshi and Cameotra, Swaranjit Singh and Mahanta, Pinakeswar and Goswami, Pranab (2009) Silk-fiber immobilized lipase-catalyzed hydrolysis of emulsified sunflower oil. Applied biochemistry and biotechnology, 157 (3). pp. 593-600. ISSN 1559-0291

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Abstract

Lipase was immobilized in silk fibers through glutaraldehyde cross-linking to a maximum loading of 59 U/g silk-fiber and the immobilized lipase was utilized for the hydrolysis of sunflower oil (Helianthus annuus). The hydrolytic activity of the lipase, which was poor in biphasic oil in water system, was increased significantly when the sunflower oil was emulsified in aqueous medium. The hydrolytic activities of the immobilized lipase were 48.73 +/- 1.26 U, 36.11 +/- 0.96 U, and nil when the substrate sunflower oil was used as emulsion created by a rhamnolipid biosurfactant, Triton X100, and ultrasonication, respectively. Although the efficiency of the immobilized lipase was less than 12% than the corresponding free lipase, the immobilized lipase could be reused for the biosurfactant-mediated hydrolysis of sunflower oil up to third cycle of the reaction. The yield of the fatty acids in the second, third, and fourth cycles were 49.45%, 22.91%, and 5.09%, respectively, of the yield obtained in the first cycle.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Springer Science.
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	28 Feb 2012 16:10
Last Modified:	28 Feb 2012 16:10
URI:	http://crdd.osdd.net/open/id/eprint/1094

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