Interaction between Vibrio mimicus and Acanthamoeba castellanii.

Abd, Hadi and Valeru, Soni Priya and Sami, Susan Marouf and Saeed, Amir and Raychaudhuri, Saumya and Sandström, Gunnar (2010) Interaction between Vibrio mimicus and Acanthamoeba castellanii. Environmental microbiology reports, 2 (1). pp. 166-171. ISSN 1758-2229

PDF saumya2010.pdf - Published Version Restricted to Registered users only Download (315Kb)

Abstract

Vibrio mimicus is a Gram-negative bacterium, which causes gastroenteritis and is closely related to Vibrio cholerae. The environmental reservoir of this bacterium is far from defined. Acanthamoeba as well as Vibrio species are found in diverse aquatic environments. The present study was aimed to investigate the ability of A. castellanii to host V. mimicus, the role of bacterial protease on interaction with A. castellanii and to disclose the ability of cysts to protect intracellular V. mimicus. Co-cultivation, viable counts, gentamicin assay, electron microscopy and statistical analysis showed that co-cultivation of wild type and luxO mutant of V. mimicus strains with A. castellanii did not inhibit growth of the amoeba. On the other hand co-cultivation enhanced growth and survival of V. mimicus strains. Vibrio mimicus showed intracellular behaviour because bacteria were found to be localized in the cytoplasm of amoeba trophozoites and remain viable for 14 days. The cysts protected intracellular V. mimicus from high level of gentamicin. The intracellular growth of V. mimicus in A. castellanii suggests a role of A. castellanii as a host for V. mimicus.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Wiley.
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	28 Feb 2012 16:07
Last Modified:	28 Feb 2012 16:07
URI:	http://crdd.osdd.net/open/id/eprint/1116

Actions (login required)

View Item