Palladium@gold bimetallic nanostructures as peroxidase mimic for development of sensitive fluoroimmunoassay.

Nangia, Yogesh and Kumar, Bipul and Kaushal, Jyotsna and Suri, C Raman (2012) Palladium@gold bimetallic nanostructures as peroxidase mimic for development of sensitive fluoroimmunoassay. Analytica chimica acta, 751. pp. 140-5. ISSN 1873-4324

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Abstract

In this paper, gold nanoparticles coated with palladium dots (Pd@Au) bimetallic nanostructures have been reported to have a peroxidase like activity which is not found in their monometallic counterparts. Based on this finding, we have developed an immunoassay in which antibody-modified Pd@Au nanostructure catalyzes the dimerization of a fluorogenic substrate for peroxidase, 3-(4-dihydroxy phenyl) propionic acid (HPPA), to generate high fluorescence signal. Specific antibodies against bensulfuron-methyl were generated by using a well characterized bensulfuron-protein conjugate as an immunogen, and the assay was performed in a competitive immunoassay format where Pd@Au nanostructure was bound to secondary antibody to show the peroxidase like activity. The developed immunoassay exhibited an excellent sensitivity showing a dynamic response range from 0.001 to 100ngmL(-1) for herbicide bensulfuron-methyl with a detection limit of 0.01ngmL(-1) (n=3). The newly synthesized bimetallic nanostructure shows the advantages of low cost, easy synthesis and tunable catalytic activity, making it a promising substitution of enzyme peroxidase in different applications.

Item Type: Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords: Bimetallic nanoparticles; Enzyme mimics;Fluoro-immunoassay; Bensulfuron
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 31 Jan 2013 06:16
Last Modified: 22 Jul 2015 05:30
URI: http://crdd.osdd.net/open/id/eprint/1216

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