Nangia, Yogesh and Kumar, Bipul and Kaushal, Jyotsna and Suri, C Raman (2012) Palladium@gold bimetallic nanostructures as peroxidase mimic for development of sensitive fluoroimmunoassay. Analytica chimica acta, 751. pp. 140-5. ISSN 1873-4324
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Abstract
In this paper, gold nanoparticles coated with palladium dots (Pd@Au) bimetallic nanostructures have been reported to have a peroxidase like activity which is not found in their monometallic counterparts. Based on this finding, we have developed an immunoassay in which antibody-modified Pd@Au nanostructure catalyzes the dimerization of a fluorogenic substrate for peroxidase, 3-(4-dihydroxy phenyl) propionic acid (HPPA), to generate high fluorescence signal. Specific antibodies against bensulfuron-methyl were generated by using a well characterized bensulfuron-protein conjugate as an immunogen, and the assay was performed in a competitive immunoassay format where Pd@Au nanostructure was bound to secondary antibody to show the peroxidase like activity. The developed immunoassay exhibited an excellent sensitivity showing a dynamic response range from 0.001 to 100ngmL(-1) for herbicide bensulfuron-methyl with a detection limit of 0.01ngmL(-1) (n=3). The newly synthesized bimetallic nanostructure shows the advantages of low cost, easy synthesis and tunable catalytic activity, making it a promising substitution of enzyme peroxidase in different applications.
Item Type: | Article |
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Additional Information: | Copyright of this article belongs to Elsevier Science. |
Uncontrolled Keywords: | Bimetallic nanoparticles; Enzyme mimics;Fluoro-immunoassay; Bensulfuron |
Subjects: | Q Science > QR Microbiology |
Depositing User: | Dr. K.P.S.Sengar |
Date Deposited: | 31 Jan 2013 06:16 |
Last Modified: | 22 Jul 2015 05:30 |
URI: | http://crdd.osdd.net/open/id/eprint/1216 |
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