Mammalian proapoptotic factor ChaC1 and its homologues function as γ-glutamyl cyclotransferases acting specifically on glutathione.

Kumar, Akhilesh and Tikoo, Shweta and Maity, Shuvadeep and Sengupta, Shantanu and Sengupta, Sagar and Kaur, Amandeep and Kumar Bachhawat, Anand (2012) Mammalian proapoptotic factor ChaC1 and its homologues function as γ-glutamyl cyclotransferases acting specifically on glutathione. EMBO reports, 13 (12). pp. 1095-101. ISSN 1469-3178

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Abstract

ChaC1 is a mammalian proapoptic protein of unknown function induced during endoplasmic reticulum stress. We show using in vivo studies and novel in vitro assays that the ChaC family of proteins function as γ-glutamyl cyclotransferases acting specifically to degrade glutathione but not other γ-glutamyl peptides. The overexpression of these proteins (but not the catalytically dead E>Q mutants) led to glutathione depletion and enhanced apoptosis in yeast. The ChaC family is conversed across all phyla and represents a new pathway for glutathione degradation in living cells, and the first cytosolic pathway for glutathione degradation in mammalian cells.

Item Type:	Article
Additional Information:	Copyright of this article belongs to NPG.
Uncontrolled Keywords:	apoptosis; ChaC1; γ-glutamyl cyclotransferases
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	31 Jan 2013 06:07
Last Modified:	31 Jan 2013 06:07
URI:	http://crdd.osdd.net/open/id/eprint/1217

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