Mechanism of drug resistance in clonally related clinical isolates of Vibrio fluvialis isolated in Kolkata, India.

Srinivasan, Vijaya Bharathi and Virk, Rupinder Kaur and Kaundal, Amita and Chakraborty, Rupa and Datta, Basabjit and Ramamurthy, T and Mukhopadhyay, A K and Ghosh, Amit (2006) Mechanism of drug resistance in clonally related clinical isolates of Vibrio fluvialis isolated in Kolkata, India. Antimicrobial agents and chemotherapy, 50 (7). pp. 2428-32. ISSN 0066-4804

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Abstract

The molecular mechanisms of drug resistance in 19 strains of Vibrio fluvialis isolated from 1998 to 2002 in Kolkata, India, were investigated. Class 1 integrons were detected in eight strains, and four strains were found to carry SXT integrases. In the presence of carbonyl cyanide m-chlorophenylhydrazone or reserpine, all nalidixic acid- and ciprofloxacin-resistant strains became sensitive, suggesting that drug efflux plays a major role in quinolone resistance in V. fluvialis. It was further seen that strains which had MICs of > 25 microg/ml for nalidixic acid had a sense mutation (Ser to Ile) at position 83 of the quinolone resistance-determining region of gyrA. All except one of the integron- and SXT integrase-bearing strains belonged to the same ribotype.

Item Type:	Article
Additional Information:	Copyright of this article belongs to ASM.
Subjects:	Q Science > QD Chemistry
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	09 Jan 2012 04:23
Last Modified:	09 Jan 2015 08:57
URI:	http://crdd.osdd.net/open/id/eprint/147

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