A web-based resource for designing therapeutics against Ebola Virus.

Dhanda, Sandeep Kumar and Chaudhary, Kumardeep and Gupta, Sudheer and Brahmachari, Samir Kumar and Raghava, G.P.S. (2016) A web-based resource for designing therapeutics against Ebola Virus. Scientific reports, 6. p. 24782. ISSN 2045-2322

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Abstract

In this study, we describe a web-based resource, developed for assisting the scientific community in designing an effective therapeutics against the Ebola virus. Firstly, we predicted and identified experimentally validated epitopes in each of the antigens/proteins of the five known ebolaviruses. Secondly, we generated all the possible overlapping 9mer peptides from the proteins of ebolaviruses. Thirdly, conserved peptides across all the five ebolaviruses (four human pathogenic species) with no identical sequence in the human proteome, based on 1000 Genomes project, were identified. Finally, we identified peptide or epitope-based vaccine candidates that could activate both the B- and T-cell arms of the immune system. In addition, we also identified efficacious siRNAs against the mRNA transcriptome (absent in human transcriptome) of all the five ebolaviruses. It was observed that three species can potentially be targeted by a single siRNA (19mer) and 75 siRNAs can potentially target at least two species. A web server, EbolaVCR, has been developed that incorporates all the above information and useful computational tools (http://crdd.osdd.net/oscadd/ebola/).

Item Type:	Article
Additional Information:	Open Access
Uncontrolled Keywords:	Ebola Virus; Bioinformatics; Health database
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	29 Sep 2016 10:56
Last Modified:	29 Sep 2016 10:56
URI:	http://crdd.osdd.net/open/id/eprint/1862

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