siRNAmod: A database of experimentally validated chemically modified siRNAs.

Dar, Showkat Ahmad and Thakur, Anamika and Qureshi, Abid and Kumar, Manoj (2016) siRNAmod: A database of experimentally validated chemically modified siRNAs. Scientific reports, 6. p. 20031. ISSN 2045-2322

PDF (Open Access) srep20031.pdf - Published Version Download (1292Kb)

Abstract

Small interfering RNA (siRNA) technology has vast potential for functional genomics and development of therapeutics. However, it faces many obstacles predominantly instability of siRNAs due to nuclease digestion and subsequently biologically short half-life. Chemical modifications in siRNAs provide means to overcome these shortcomings and improve their stability and potency. Despite enormous utility bioinformatics resource of these chemically modified siRNAs (cm-siRNAs) is lacking. Therefore, we have developed siRNAmod, a specialized databank for chemically modified siRNAs. Currently, our repository contains a total of 4894 chemically modified-siRNA sequences, comprising 128 unique chemical modifications on different positions with various permutations and combinations. It incorporates important information on siRNA sequence, chemical modification, their number and respective position, structure, simplified molecular input line entry system canonical (SMILES), efficacy of modified siRNA, target gene, cell line, experimental methods, reference etc. It is developed and hosted using Linux Apache MySQL PHP (LAMP) software bundle. Standard user-friendly browse, search facility and analysis tools are also integrated. It would assist in understanding the effect of chemical modifications and further development of stable and efficacious siRNAs for research as well as therapeutics. siRNAmod is freely available at: http://crdd.osdd.net/servers/sirnamod.

Item Type:	Article
Additional Information:	Open Access
Uncontrolled Keywords:	Genetic databases;Predictive medicine;RNAi
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	04 Oct 2016 06:18
Last Modified:	04 Oct 2016 06:18
URI:	http://crdd.osdd.net/open/id/eprint/1919

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