Gaurav, Sachin and Ranjan, Rajiv Kumar and Kuldeep, Jitendra and Dhiman, Kanika and Mahapatra, Pinaki Prasad and Ashish, . and Siddiqi, Mohammad Imran and Ahmed, Shakil (2021) The N-terminus region of Drp1, a Rint1 family protein is essential for cell survival and its interaction with Rad50 protein in fission yeast S.pombe. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1865 (1).
Full text not available from this repository. (Request a copy)Abstract
Background: Defects in DNA repair pathway can lead to double-strand breaks leading to genomic instability. Earlier we have shown that S.pombe Drp1, a Rint1/Tip1 family protein is required for the recovery from DNA damage. Methods: Various truncations of Drp1 protein were constructed and their role in DNA damage response and interaction with Rad50 protein has been studied by co-immunoprecipitation and pull-down assays. Results: The structural and functional analysis of Drp1 protein revealed that the N-terminus region of Drp1 is indispensable for the survival. The C-terminus truncation mutants, drp1 Cid and drp1C24 exhibit temperature sensitive phenotype and are hypersensitive against DNA damaging agents with elevated level of Rad52-YFP foci at non-permissive temperature indicating the impairment for DNA damage repair pathway. The essential N-terminus region of Drp1 interacts with the C-terminus region of Rad50 and might be involved in influencing the MRN/X function. Small-angle X-ray (SAXS) analysis revealed three-domain like shapes in Drp1 protein while the C-terminus region of Rad50 exhibit unusual bulges. Computational docking studies revealed the amino acid residues at the C-terminus region of Rad50 that are involved in the interaction with the residues present at the Nterminal region of Drp1 indicating the importance of the N-terminal region of Drp1 protein. Conclusions: We have identified the region of Drp1 and Rad50 proteins that are involved in the interaction and their role in the DNA damage response pathway has been analyzed. General significance: The functional and structural aspects of fission yeast Drpl protein and its interaction with Rad50 have been elucidated.
Item Type: | Article |
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Additional Information: | The copyright of this article belongs to Elsevier Science. |
Uncontrolled Keywords: | S.pombe;Drp1;Rint1;Rad50DNA; repair;SAXS analysis |
Subjects: | Q Science > QR Microbiology |
Depositing User: | Dr. K.P.S.Sengar |
Date Deposited: | 01 Feb 2021 04:09 |
Last Modified: | 01 Feb 2021 04:09 |
URI: | http://crdd.osdd.net/open/id/eprint/2647 |
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