Srivastava, Suchita and Kaur, Satinderdeep and Verma, Hemant Kumar and Rani, Suman and Thakur, Manisha and Haldar, Swati and Singh, Jagmohan (2021) Reciprocal relation between reporter gene transcription and translation efficiency in fission yeast. Plasmid, 115. ISSN 1095-9890
Full text not available from this repository. (Request a copy)Abstract
The fission yeast, Schizosaccharomyces pombe, is an excellent model for basic research but is not useful for commercial scale protein expression due to lack of strong expression vectors. Earlier, we showed that the lsd90 promoter elicited significantly greater GFP expression level than the adh1 and nmt1 promoters, albeit in different vector backbones. Here, we have systematically investigated the contribution of selectable markers, LEU2 and URA3m to GFP expression: while LEU2 elicited very low expression, the URA3m gene, with truncated promoter, elicited much greater GFP expression level with all promoters. Paradoxically, an inverse correlation was observed between the GFP transcription and translation efficiency. This system can be useful for understanding the factors governing recombinant gene expression and optimization of protein production.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Elsevier Science/Academic Press |
| Uncontrolled Keywords: | Reporter gene; Promoter; Selectable marker; Transcription; Translation; GFP |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 29 Jun 2021 07:28 |
| Last Modified: | 29 Jun 2021 07:28 |
| URI: | http://crdd.osdd.net/open/id/eprint/2697 |
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