Kaur , Bhupinder and Kumar, Nitish and Chawla, Sonam and Sharma, Deepika and Korpole , Suresh and Sharma, Rajni and Patel, Manoj K and Chopra, Kanwaljit and Chaurasia, Om Prakash and Saxena, Shweta (2022) A comparative study of in-vitro and in-silico anti-candidal activity and GC-MS profiles of snow mountain garlic vs. normal garlic. Journal of applied microbiology. ISSN 1364-5072
Full text not available from this repository. (Request a copy)Abstract
Aim: The study aimed to profile the volatile phytocomposition of snow mountain garlic (SMG) compared to normal garlic and investigate the anti-Candida efficacy against clinically relevant multi-drug resistant isolates of Candida species. Methods and results: Herein, SMG has shown significantly superior fungicidal power at 2x-MIC dose against C. albicans and C. glabrata in killing kinetic evaluation unlike the fungistatic effect of normal garlic. GC-MS headspace-based profiling of SMG showed 5 unique volatile compounds and a 5-fold higher content of saponins than normal garlic. In an in-silico analysis, cholesta-4,6-dien-3-ol,(3-beta) was uniquely identified in SMG as a potential inhibitor with high binding affinity to the active site of exo-1,3-betaglucan synthase, an established anti-candida drug target crucial for the biofilm matrix formation, thus suggesting a plausible anti-Candida mechanism. Conclusion: The in-vitro and in-silico studies have demonstrated the Candida-cidal and anti-biofilm activities of SMG, distinguishing it from the Candida-static efficacy of normal garlic.
| Item Type: | Article |
|---|---|
| Additional Information: | The copyright of this article belongs to Wiley |
| Uncontrolled Keywords: | Candida-cidal activity; anti-fungal drug resistance; exo-1,3 beta glucan synthase; phytoactive compounds; snow mountain garlic |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 22 Jul 2022 04:47 |
| Last Modified: | 22 Jul 2022 04:47 |
| URI: | http://crdd.osdd.net/open/id/eprint/2979 |
Actions (login required)
 |
View Item |
