Choudhary, Megha and Bisht, Bhawana and Saini, Jai Kumar and Bharti, . and Singh, Pargat and Bhardwaj, Priya and Dilawari, Rahul and Pinnaka, Anil Kumar and Ray, Pallab and Gupta, Madhu and Sethi, Sunil and Suri, C Raman and Raje, Manoj and Bhalla, Vijayender (2023) Bifunctionalized nanobioprobe based rapid color-shift assay for typhoid targeting Vi capsular polysaccharide. Biosensors & bioelectronics, 228.
Full text not available from this repository. (Request a copy)Abstract
Typhoid fever is an acute illness caused by Salmonella Typhi and the current diagnostic gap leads to inaccurate, over-diagnosis of typhoid leading to excessive use of antibiotics. Herein, to address the challenges we describe a new rapid color-shift assay based on a novel bifunctional nanobioprobe (Vi-AgNP probe) that is functionalized with specific biomarker Vi polysaccharide and also has the co-presence of Ag as urease inhibitor. The immunoreactions between the Vi with specific antibodies (Abs) present in typhoid patient sample forms a shielding barrier over Vi-AgNP probe rendering the urease to be active, generating colored output. Vi polysaccharide coating on the AgNP was visualized using HRTEM. TEM was performed to get insight into shielding barrier formation by the Abs. MST (microscale thermophoresis) data showed less binding Kd of 7.43 μM in presence of Abs whereas probe with urease showed efficient binding with Kd 437 nM. The assay was validated using 53 human sera samples and proven effective with 100% sensitivity. The assay showed relative standard deviation (RSD) of 4.3% estimated using rabbit anti-Vi Abs. The entire procedure could be completed within 15 min. Unlike lateral flow based assays, our assay does not require multiple combination of Abs for detection. The assay format was also found compatible in paper strip test that provides promising opportunities to develop low-cost on-spot assay for clinical diagnostics.
| Item Type: | Article |
|---|---|
| Additional Information: | The copyright of this article belongs to Elsevier Science |
| Uncontrolled Keywords: | Bifunctional probe; Colorimetric detection; Sero-diagnosis; Thiolated Vi polysaccharide; Vi polysaccharide capped AgNP. |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 07 Jun 2023 07:27 |
| Last Modified: | 07 Jun 2023 07:27 |
| URI: | http://crdd.osdd.net/open/id/eprint/3079 |
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