Evidence that phosphate specific transporter is amplified in a fluoroquinolone resistant Mycobacterium smegmatis.

Bhatt, K and Banerjee, S K and Chakraborti, Pradip K (2000) Evidence that phosphate specific transporter is amplified in a fluoroquinolone resistant Mycobacterium smegmatis. European journal of biochemistry / FEBS, 267 (13). pp. 4028-32. ISSN 0014-2956

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Abstract

We reported in an earlier study that active efflux of drug has a predominant role in conferring resistance in a laboratory-generated ciprofloxacin-resistant mutant of Mycobacterium smegmatis. This mutant exhibited mRNA level overexpression, as well as chromosomal amplification, of the gene pstB, encoding the putative ATPase subunit of phosphate specific transport (Pst) system. We demonstrate here that this mutant shows enhanced phosphate uptake and that inactivation of pstB in the parental strain results in loss of high affinity phosphate uptake and hypersensitivity to fluoroquinolones. These findings suggest a novel role of the Pst system in active efflux, in addition to its involvement in phosphate transport.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Wiley.
Uncontrolled Keywords:	ABC transporter; drug resistance; fluoroquinolone; mycobacteria; phosphate specific transporter.
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	04 Jan 2012 15:01
Last Modified:	29 Mar 2012 07:18
URI:	http://crdd.osdd.net/open/id/eprint/316

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