Sinha, Anunay and Khosla, Sanjeev (2024) Epigenetic landscape of intestinal cell line HT29 cocultured with Lacticaseibacillus. Epigenomics, 16 (15-16). pp. 1081-1096.
Full text not available from this repository.Abstract
Aim: To investigate the changes in epigenetic landscape of HT29 cells upon coculture with the Lacticaseibacillus.Materials & methods: Histone and m6A mRNA modifications were examined by biochemical and NGS-based methods including western blotting, colorimetric assays, ChIP-Seq and direct mRNA sequencing. LC-MS was performed to identify Lacticaseibacillus secretome.Results: In cocultured HT29 cells global enrichment of H3K9ac and H3K4me3 and depletion of H3K9me3 mark was observed; mean genic positional signals showed depletion of H3K9ac and H3K4me3 at the TSS but enrichment in the upstream region; m6A methylation was altered in mRNAs corresponding to specific gene pathways; Lacticaseibacillus HU protein interacts with histone H3.Conclusion: Lacticaseibacillus can epigenetically alter specific genetic pathways in human intestinal cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | epigenetics; histone modifications; host-microbe Interaction; lactobacillus; m6A methylation |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 07 Feb 2026 11:55 |
| Last Modified: | 07 Feb 2026 11:55 |
| URI: | http://crdd.osdd.net/open/id/eprint/3349 |
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