Construction of a recombinant live oral vaccine from a non-toxigenic strain of Vibrio cholerae O1 serotype inaba biotype E1 Tor and assessment of its reactogenicity and immunogenicity in the rabbit model.

Thungapathra, M and Sharma, C and Gupta, N and Ghosh, R K and Mukhopadhyay, A and Koley, H and Nair, G B and Ghosh, A (1999) Construction of a recombinant live oral vaccine from a non-toxigenic strain of Vibrio cholerae O1 serotype inaba biotype E1 Tor and assessment of its reactogenicity and immunogenicity in the rabbit model. Immunology letters, 68 (2-3). pp. 219-27. ISSN 0165-2478

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Abstract

The disease cholera is an important cause of mortality in many developing countries. Though it can be controlled through improved sanitation, this goal is not easily attainable in many countries. Development of an efficacious vaccine offers the best immediate solution. A new oral candidate vaccine has been constructed from a non-toxigenic strain of Vibrio cholerae E1 Tor, Inaba, which is not only devoid of the cholera toxin (CT) virulence cassette but also is completely non-reactogenic in rabbit ileal loop assay. The strain, however, had toxR and tcpA genes. Through a series of manipulations, the ctxB gene of V. cholerae, responsible for the production of the 'B' subunit of the cholera toxin (CTB) was introduced into the cryptic hemolysin locus of the strain. The resulting strain, named vaccine attempt 1.3 (VA1.3), was found to be able to produce copious amounts of CTB. In the RITARD model this strain was found to be non-reactogenic and provided full protection against the challenge doses of both V. cholerae O1, classical and E1 Tor. In the immunized rabbit it invoked significant levels of anti-bacterial and anti-toxin immunity.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science
Uncontrolled Keywords:	Cholera toxin; Non-toxinogenic strain; Oral vaccine
Subjects:	Q Science > QR Microbiology > QR180 Immunology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	03 Jan 2012 16:49
Last Modified:	03 Jan 2012 16:49
URI:	http://crdd.osdd.net/open/id/eprint/341

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