A 150-kDa molecule of macrophage membrane stimulates interleukin-2 and interferon-gamma production and proliferation of ovalbumin-specific CD4+ T cells.

Agrewala, J N and Vinay, D S and Joshi, A and Mishra, G C (1994) A 150-kDa molecule of macrophage membrane stimulates interleukin-2 and interferon-gamma production and proliferation of ovalbumin-specific CD4+ T cells. European journal of immunology, 24 (9). pp. 2092-7. ISSN 0014-2980

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Abstract

In the present study, we describe the potential co-stimulatory role of a macrophage membrane-associated protein of 150 kDa (M150). The protein was isolated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was found to be a single molecule on two-dimensional gel electrophoresis. The molecule was re-constituted in phosphatidyl choline vesicles and tested for its ability to promote the proliferation and the secretion of lymphokines from T helper (Th) cells. The reconstituted M150 induced a significant proliferation of anti-CD3 monoclonal antibody (mAb)-stimulated ovalbumin-specific CD4+ T cells. Further, Th cells activated with this molecule in the presence of anti-CD3 mAb mainly secreted interleukin (IL)-2 and interferon-gamma but not IL-4. M150 could not promote the proliferation of Th cells, or lymphokine secretion in the absence of anti-CD3 mAb. These observations suggest that M150 acts by selectively activating a Th1-like immune response.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Wiley
Subjects:	Q Science > QR Microbiology > QR180 Immunology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	13 Dec 2011 16:51
Last Modified:	27 Mar 2014 09:21
URI:	http://crdd.osdd.net/open/id/eprint/405

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