Temperature dependence of transcriptional activity of yeast 3-phosphoglycerokinase promoter in Escherichia coli.

Mondal, Alok K and Roy, T K (1990) Temperature dependence of transcriptional activity of yeast 3-phosphoglycerokinase promoter in Escherichia coli. Biochemical and biophysical research communications, 170 (1). pp. 1-9. ISSN 0006-291X

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Abstract

We have studied the activity of yeast PGK promoter in E. coli at different temperatures using lacZ as the reporter gene. The B-galactosidase activity was found to be less at 42 degrees C than at 30 degrees C. Northern blot analysis showed that the level of lacZ transcript was less in recombinant cells grown at 42 degrees C, whereas plasmid copy number per cell was more as compared to recombinant cells grown at 30 degrees C. Data suggest that the yeast PGK promoter is less active at 42 degrees C and that this activity is regulated at the level of transcription.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science
Subjects:	Q Science > QD Chemistry
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	12 Dec 2011 15:39
Last Modified:	09 Jan 2015 09:03
URI:	http://crdd.osdd.net/open/id/eprint/433

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