Reductive dehalogenation mediated initiation of aerobic degradation of 2-chloro-4-nitrophenol (2C4NP) by Burkholderia sp. strain SJ98.

Pandey, Janmejay and Heipieper, Hermann J and Chauhan, Archana and Arora, Pankaj Kumar and Prakash, Dhan and Takeo, Masahiro and Jain, R K (2011) Reductive dehalogenation mediated initiation of aerobic degradation of 2-chloro-4-nitrophenol (2C4NP) by Burkholderia sp. strain SJ98. Applied microbiology and biotechnology, 92 (3). pp. 597-607. ISSN 1432-0614

[img] PDF
jain11.pdf - Published Version
Restricted to Registered users only

Download (400Kb) | Request a copy
Official URL: http://www.springerlink.com/content/p6378j38472161...

Abstract

Burkholderia sp. strain SJ98 (DSM 23195) was previously isolated and characterized for degradation and co-metabolic transformation of a number nitroaromatic compounds. In the present study, we evaluated its metabolic activity on chlorinated nitroaromatic compounds (CNACs). Results obtained during this study revealed that strain SJ98 can degrade 2-chloro-4-nitrophenol (2C4NP) and utilize it as sole source of carbon, nitrogen, and energy under aerobic conditions. The cells of strain SJ98 removed 2C4NP from the growth medium with sequential release of nearly stoichiometric amounts of chloride and nitrite in culture supernatant. Under aerobic degradation conditions, 2C4NP was transformed into the first intermediate that was identified as p-nitrophenol by high-performance liquid chromatography, LCMS-TOF, and GC-MS analyses. This transformation clearly establishes that the degradation of 2C4NP by strain SJ98 is initiated by "reductive dehalogenation"; an initiation mechanism that has not been previously reported for microbial degradation of CNAC under aerobic conditions.

Item Type: Article
Additional Information: Copyright of this article belongs to Springer Science.
Uncontrolled Keywords: Chlorinated nitroaromatic compounds . Burkholderia . Aerobic degradation
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 09 Dec 2011 06:55
Last Modified: 09 Jan 2015 11:14
URI: http://crdd.osdd.net/open/id/eprint/466

Actions (login required)

View Item View Item