Optimization of culture conditions for the production of rifamycin oxidase by Curvularia lunata

Banerjee, U C (1994) Optimization of culture conditions for the production of rifamycin oxidase by Curvularia lunata. World Journal of Microbiology & Biotechnology, 10 (4). pp. 462-464. ISSN 0959-3993

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Abstract

Maximum activity (8.9 IU/ml) of rifamycin oxidase in Curvularia lunata, grown in shake-flask culture at 28°C and pH 6.5, was after 96 h. Nearly all the glucose was used in 72 h. An initial culture pH of 6.5 and 28°C were optimum for the growth and enzyme production. Among various carbon and organic nitrogen sources, carboxymethylcellulose and peptone were the most effective for enzyme yield. The rate of enzyme production was enhanced when yeast extract was also added to the medium. The optimum medium for the production of rifamycin oxidase contained 10 g each of yeast extract, peptone and carboxymethylcellulose/l and 0.04% (NH4)2SO4.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Springer Science.
Uncontrolled Keywords:	Carboxymethylcellulose - Curvularia lunata - optimization - rifamycin oxidase
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	27 Jan 2012 15:37
Last Modified:	28 Mar 2014 10:58
URI:	http://crdd.osdd.net/open/id/eprint/715

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