Influence of Macromixing on Plasmid Stability during Batch Fer-mentation with Recombinant Bacteria.

Patnaik, P R (1997) Influence of Macromixing on Plasmid Stability during Batch Fer-mentation with Recombinant Bacteria. INDIAN JOURNAL OF CHEMICAL TECHNOLOGY , 4 (6). pp. 291-297. ISSN 0971-457X

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Abstract

Macromixing in a batch bioreactor has been analysed through an extended version of a two-region model proposed earlier. Two internal dilution rates characterise the degree of macromixing. Computations for an Escherichia coli culture containing the plasmid pBR Eco gap show that in order to maximise the average concentration (or mass fraction) of plasmid-containing cells, the region into which the inoculum is introduced should be poorly (or perfectly) mixed while the other region should be perfectly (or poorly) mixed. Time-domain profiles for the two regions suggest that plasmid-containing cells become homogeneously distributed faster than plasmid-free cells. Comparison of the regional profiles for the cases of the largest average concentration and the largest-average mass fraction df plasmid-containing cells reveal differences which indicate that a partially macromixed bioreactor may be an optimal choice.

Item Type: Article
Additional Information: Copyright of this article belongs to NISCAIR.
Subjects: Q Science > QD Chemistry
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 27 Jan 2012 15:05
Last Modified: 27 Mar 2014 06:38
URI: http://crdd.osdd.net/open/id/eprint/761

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