Characterization of a fungal amylase from Mucor sp. associated with the marine sponge Spirastrella sp.

Mohapatra, B.R. and Banerjee, U C and Bapuji, M (1998) Characterization of a fungal amylase from Mucor sp. associated with the marine sponge Spirastrella sp. Journal of Biotechnology, 60 (1-2). pp. 113-117. ISSN 01681656

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Abstract

A novel amylase was isolated from the Mucor sp. associated with the marine sponge Spirastrella sp., grown at 30°C. The enzyme has an optimum pH of 5.0 and an optimum temperature of 60°C. The half lives of the partially purified enzyme at 55 and 60°C were 120 and 50 min, respectively. The activation and deactivation energies of the partially purified enzyme were 46.60 and 157.05 kJ mol−1, respectively. The enzyme activity was not affected by the addition of 3% NaCl, 10 mM Ca2+ and 25 mM Mg2+, but was strongly inhibited by EDTA.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords:	Activation and deactivation energies; Amylase; Mucor sp.; Spirastrella sp.; Thermostability
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	30 Jan 2012 17:57
Last Modified:	09 Jan 2015 08:47
URI:	http://crdd.osdd.net/open/id/eprint/783

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