Improvement of the microbial production of streptokinase by controlled filtering of process noise

Patnaik, P R (1999) Improvement of the microbial production of streptokinase by controlled filtering of process noise. Process Biochemistry, 35 (3-4). pp. 309-315. ISSN 13595113

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Abstract

Disturbances during operation are an ubiquitous feature of large-scale fermentations. This kind of process noise is often describable by a Gaussian distribution and is considered undesirable but unavoidable. In this paper, the fed-batch production of streptokinase (SK) was studied when such disturbances occurred in the inflow rate of the substrate. As the variance of the noise increased upto 6% of the instantaneous value of the flowrate, there was a gradual improvement in the SK activity, after which the activity decreased for larger variances. The peak activity (10 850 U/ml) was 22% higher than for a smooth noise-free feed. Similar earlier observations for β-galactosidase and β-lactamase suggest that normal disturbances may be harnessed to enhance fermentation efficiency by filtering them such that they are maintained within an optimal range.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords:	Streptokinase fermentation; Inflow noise; Process improvement; Controlled filtering
Subjects:	Q Science > QD Chemistry
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	30 Jan 2012 17:53
Last Modified:	09 Jan 2015 10:32
URI:	http://crdd.osdd.net/open/id/eprint/804

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