Samanta, S K and Jain, R K (2000) Evidence for plasmid-mediated chemotaxis of Pseudomonas putida towards naphthalene and salicylate. Canadian journal of microbiology, 46 (1). pp. 1-6. ISSN 0008-4166
Full text not available from this repository. (Request a copy)Abstract
A naphthalene (Nap) and salicylate (Sal) degrading microorganism, Pseudomonas putida RKJ1, is chemotactic towards these compounds. This strain carries a 83 kb plasmid. A 25 kb EcoRI fragment of the plasmid contains the genes responsible for Nap degradation through Sal. RKJ5, the plasmid-cured derivative of RKJ1, is neither capable of degradation nor is chemotactic towards Nap or Sal. The recombinant plasmid pRKJ3, which contained a 25 kb EcoRI fragment, was transferred back into the plasmid-free wild-type strain RKJ5, and the transconjugant showed both degradation and chemotaxis. The recombinant plasmid pRKJ3 was also transferred into motile, plasmid-free P. putida KT2442. The resulting transconjugant (RKJ15) showed chemotaxis towards both Nap and Sal. Two mutant strains carrying deletions in pRKJ3 (in KT2442) with phenotypes Nap- Sal+ and Nap- Sal-, were also tested for chemotaxis. It was found that the Nap- Sal+ mutant strain showed chemotaxis towards Sal only, whereas the Nap- Sal- mutant strain is non-chemotactic towards both the compounds. These results suggest that the metabolism of Nap and Sal may be required for the chemotactic activity.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to National Research Council. |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 02 Feb 2012 16:31 |
| Last Modified: | 02 Feb 2012 16:31 |
| URI: | http://crdd.osdd.net/open/id/eprint/850 |
Actions (login required)
 |
View Item |
