Artificial neural network based B-cell epitope prediction server

                                                        

Instructions



In order to use the ABCpred server for prediction on amino acid sequences:
  1. (optional) Enter a name for the sequence.

  2. Enter the sequence in the sequence window (with no header line), or give a file name.

    The sequence must be written using the one letter amino acid code: `acdefghiklmnpqrstvwy' or `ACDEFGHIKLMNPQRSTVWY'.
    Other letters will be converted to `X' and treated as unknown amino acids.
    Other characters, such as whitespace and numbers, will simply be ignored.

  3. Choose the output format: Graphical or Tabular, the default is Graphical.

  4. Change the threshold [+0.1 to +1.0]: increase in the threshold results in better specificity, but worse sensitivity. At 0.5, it shows 65.93% accruacy with equal sensitivity and specificity using window length of 16.

  5. Select the window length (10,12,14,16,18,20), default length is 16.

  6. Press the "Submit sequence" button.

  7. A WWW page will return the results when the prediction is ready. Response time depends on system load.
Contact :  G.P.S. Raghava