NPACT- Naturally occuring Plant based Anticancerous Compound-Activity-Target DataBase
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Compound: Butein

TargetsGene NameGene IDCancerCell LinesIC50ED50EC50GI50RemarkReferences
BAXBCL2-associated X protein581Prostate CancerLNCaP----The deregulation of apoptosis is studied by the expression of Bax and Bcl2. Bax is proapoptotic protein, there was a significant increase in the protein expression of Bax with a dose-dependent on treatment of LNCaP and CWR22Rm1 cells with butein.22114764
BAXBCL2-associated X protein581Prostrate CarcinomaCWR22Rm1----The deregulation of apoptosis is studied by the expression of Bax and Bcl2. Bax is proapoptotic protein, there was a significant increase in the protein expression of Bax with a dose-dependent on treatment of LNCaP and CWR22Rm1 cells with butein.22114764
BAXBCL2-associated X protein581Human promyelocytic leukemiaHL-60----The treatment of HL-60 cells with butein increased the expression of Bax protein11393587
BCL2B-cell CLL/lymphoma 2596Prostate CancerLNCaP----The deregulation of apoptosis is studied by the expression of Bax and Bcl2. Bcl2 is anti-apoptotic, there was a significant decrease in the protein expression of Bcl2 on treatment of LNCaP and CWR22Rm1 cells with butein.22114764
BCL2B-cell CLL/lymphoma 2596Prostrate CarcinomaCWR22Rm1----The deregulation of apoptosis is studied by the expression of Bax and Bcl2. Bcl2 is anti-apoptotic, there was a significant decrease in the protein expression of Bcl2 on treatment of LNCaP and CWR22Rm1 cells with butein.22114764
BCL2B-cell CLL/lymphoma 2596Human promyelocytic leukemiaHL-60----The treatment of HL-60 cells with butein decreased the expression of Bcl-2 protein11393587
CASP3caspase 3, apoptosis-related cysteine peptidase836Human promyelocytic leukemiaHL-60----The treatment of the cells with butein increased the caspase-3 activity11393587
CASP3caspase 3, apoptosis-related cysteine peptidase836Prostate CancerLNCaP----Treatment of LNCaP cells with butein (10-30 µM) caused an increase in the activation of caspases-3, -8, and -9 dose dependently.22114764
CASP8caspase 8, apoptosis-related cysteine peptidase841Prostate CancerLNCaP----Treatment of LNCaP cells with butein (10-30 µM) caused an increase in the activation of caspase-8 dose dependently.22114764
CASP9caspase 9, apoptosis-related cysteine peptidase842Prostate CancerLNCaP----Treatment of LNCaP cells with butein (10-30 µM) caused an increase in the activation of caspase-9 dose dependently.22114764
CCND1cyclin D1595Prostate CancerLNCaP----LNCaP cells with butein (10-30 µM; 48 h) caused a dose-dependent decrease in the protein expression of cyclinsD1 by using immunoblot analysis. Treatment of CWR22Rm1 cells with butein (10-30 µM; 48 h) also caused an inhibition of cyclin D1,suggesting that the effects of butein on prostrate cancer(PCa) cells are not limited for one-cell type.22114764
CCND1cyclin D1595Prostrate CarcinomaCWR22Rm1----LNCaP cells with butein (10-30 µM; 48 h) caused a dose-dependent decrease in the protein expression of cyclinsD1 by using immunoblot analysis. Treatment of CWR22Rm1 cells with butein (10-30 µM; 48 h) also caused an inhibition of cyclin D1,suggesting that the effects of butein on prostrate cancer(PCa) cells are not limited for one-cell type.22114764
CCND2Cyclin D2894Prostate CancerLNCaP----LNCaP cells with butein (10-30 µM; 48 h) caused a dose-dependent decrease in the protein expression of cyclin D2 by using immunoblot analysis.22114764
CCNE1cyclin E1898Prostate CancerLNCaP----LNCaP cells with butein (10-30 µM; 48 h) caused a dose-dependent decrease in the protein expression of cyclin E by using immunoblot analysis.22114764
CDK2cyclin-dependent kinase 21017Prostate CancerLNCaP----LNCaP cells with butein (10-30 µM; 48 h) caused a dose-dependent decrease in the protein expression of cdk2 using immunoblot analysis. Treatment of CWR22Rm1 cells with butein (10-30 µM; 48 h) also caused an inhibition of cdk 2,suggesting that the effects of butein on prostrate cancer(PCa) cells are not limited for one-cell type.22114764
CDK2cyclin-dependent kinase 21017Prostrate CarcinomaCWR22Rm1----LNCaP cells with butein (10-30 µM; 48 h) caused a dose-dependent decrease in the protein expression of cdk2 using immunoblot analysis. Treatment of CWR22Rm1 cells with butein (10-30 µM; 48 h) also caused an inhibition of cdk 2,suggesting that the effects of butein on prostrate cancer(PCa) cells are not limited for one-cell type.22114764
CDK4cyclin-dependent kinase 41019Prostate CancerLNCaP----LNCaP cells with butein (10-30 µM; 48 h) caused a dose-dependent decrease in the protein expression of cdk4 using immunoblot analysis.22114764
CDK6cyclin-dependent kinase 61021Prostate CancerLNCaP----LNCaP cells with butein (10-30 µM; 48 h) caused a dose-dependent decrease in the protein expression of cdk6 using immunoblot analysis.22114764
CDKN1Acyclin-dependent kinase inhibitor 1A (p21, Cip1)1026Prostate CancerLNCaP----Treatment with butein resulted in an induction of WAF1/p21 even at the lowest concentration of 10 lM with a significant increase at the highest concentration of 30 µM. Treatment of CWR22Rm1 cells with butein (10-30 µM; 48 h) also caused an increase in WAF1/p21, suggesting that the effects of butein on prostrate cancer(PCa) cells are not limited for one-cell type.22114764
CDKN1Bcyclin-dependent kinase inhibitor 1B (p27, Kip1)1027Prostate CancerLNCaP----Treatment with butein resulted in an induction of KIP1/p27 even at the lowest concentration of 10 lM with a significant increase at the highest concentration of 30 µM. Treatment of CWR22Rm1 cells with butein (10-30 µM; 48 h) also caused an increase in KIP1/p27, suggesting that the effects of butein on prostrate cancer(PCa) cells are not limited for one-cell typ.22114764
CHUKconserved helix-loop-helix ubiquitous kinase1147Prostate CancerLNCaP----IKKalpha protein level was measured to evaluate the possible inhibitory mechanism of butein on IkBalpha protein degradation. Immunoblot analysis showed that pretreatment of LNCaP cells with butein inhibited IKKa in a dose-dependent manner.22114764
GRB2growth factor receptor-bound protein 22885Liver Carcinoma SK-HEP-1----Western blotting analysis indicated that butein decreased the levels of GRB2 in SK-HEP-1 cell.21770460
IL6interleukin 63569Murine monocyte/macrophage line derived from ascitic tumour induced with Abelson leukaemai virusRaw2640.9 µM ---IL-6 production in LPS-stimulated RAW264 cells was found to be suppressed by butein.21372424
JUNjun proto-oncogene3725Liver Carcinoma SK-HEP-1----Western blotting analysis indicated that butein decreased the levels of MMP-2, -7, and -9, uPA, Ras, Rho A, ROCK1, ERK1/2,JNK1/2, p-p38, and p-c-Jun in SK-HEP-1 cell.21770460
MAPK1mitogen-activated protein kinase 15594Liver Carcinoma SK-HEP-1----Western blotting analysis indicated that butein decreased the levels of p38 in SK-HEP-1 cells. And butein at 62.5 and 125 µM suppressed the p38 activity.21770460
MAPK8mitogen-activated protein kinase 85599Liver Carcinoma SK-HEP-1----Western blotting analysis indicated that butein decreased the levels of JNK1/2 in SK-HEP-1 cells. Butein at 15.6-125 µM inhibited the ERK and JNK activities.21770460
MMP7matrix metallopeptidase 7 (matrilysin, uterine)4316Liver Carcinoma SK-HEP-1----The gelatin zymography assay indicated that butein inhibited the activity of matrix metalloproteinases 2 (MMP-2). Gelationlytic activity of MMP-2 in the zymogram was inhibited by butein in a dose-dependent manner. Thus, MMP 2 was down-regulated in butein-treated SK-HEP-1 cells.21770460
MMP7matrix metallopeptidase 7 (matrilysin, uterine)4316Liver Carcinoma SK-HEP-1----Western blotting analysis indicated that butein decreased the level of MMP-7 in SK-HEP-1 cell.21770460
MMP9matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)4318Liver Carcinoma SK-HEP-1----The gelatin zymography assay indicated that butein inhibited the activity of matrix metalloproteinases 9 (MMP-9). Gelationlytic activity of MMP-9 in the zymogram was inhibited by butein in a dose-dependent manner. Thus, bothMMP -9 was down-regulated in butein-treated SK-HEP-1 cell.21770460
NFKB1nuclear factor of kappa light polypeptide gene enhancer in B-cells 14790Liver Carcinoma SK-HEP-1----Butein at 50 µM might inhibit theNF-kB activity, which is able to involve cell migration and invasion of SK-HEP-1 cells.21770460
NFKB1nuclear factor of kappa light polypeptide gene enhancer in B-cells 14790Prostate CancerLNCaP----It was found that treatment with butein on LNCaP cells resulted in an inhibition of NF-kappaBp65. NF-kappaB forms a family of transcription factors that contributes in numerous biological processes, including immune response, inflammation, cell growth, survival, and development.22114764
NFKBIAnuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha 4792Prostate CancerLNCaP----A significant dose-dependent inhibition in the phosphorylation of IkBalpha protein on treatment of LNCaP cells with butein.22114764
NOS2nitric oxide synthase 2, inducible4843Murine monocyte/macrophage line derived from ascitic tumour induced with Abelson leukaemai virusRaw2645±2 µM---suppressed the TNF-a production in LPS-stimulated RAW264 cell.21372424
PARP1poly (ADP-ribose) polymerase 1142Prostate CancerLNCaP----On treatment of LNCaP cells with butein, they found that there was an increase in the protein expression of the 85 KD fragment of PARP, while there was a decrease in the protein expression of full-size PARP (116 KD) protein. And PARP has several cellular functions including DNA recombination and repair, cellular proliferation, apoptosis in ischemic conditions, and necrotic cell deat.22114764
PLAUplasminogen activator, urokinase5328Liver Carcinoma SK-HEP-1----Western blotting analysis indicated that butein decreased the level of uPA in SK-HEP-1 cells.21770460
PTK2PTK2 protein tyrosine kinase 25747Liver Carcinoma SK-HEP-1----Western blotting analysis indicated that butein decreased the levels of FAK in SK-HEP-1 cell.21770460
RHOAras homolog family member A387Liver Carcinoma SK-HEP-1----Western blotting analysis indicated that butein decreased the levels of Rho A in SK-HEP-1 cells.21770460
ROCK1Rho-associated, coiled-coil containing protein kinase 16093Liver Carcinoma SK-HEP-1----Western blotting analysis indicated that butein decreased the levels of ROCK1 in SK-HEP-1 cells.21770460
SOS1son of sevenless homolog 1 (Drosophila)6654Liver Carcinoma SK-HEP-1----Western blotting analysis indicated that butein decreased the levels of SOS1 in SK-HEP-1 cells.21770460
TNFtumor necrosis factor7124Murine monocyte/macrophage line derived from ascitic tumour induced with Abelson leukaemai virusRaw2648±4 µM---TNF-a suppressive alkaloids also inhibited NO production in RAW264 cell.21372424
VEGFAvascular endothelial growth factor A7422Liver Carcinoma SK-HEP-1----Western blotting analysis indicated that butein decreased the level of VEGF in SK-HEP-1 cells.21770460

 






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