NPACT- Naturally occuring Plant based Anticancerous Compound-Activity-Target DataBase
a

Compound: Eupafolin

TargetsGene NameGene IDCancerCell LinesIC50ED50EC50GI50RemarkReferences
BCL2B-cell CLL/lymphoma 2596Cervix AdenocarcinomaHeLa----Bcl-2 family proteins are known to control apoptosis that occurs via the mitochondrial pathway by maintaining a balance between pro- and anti-apoptotic members. The levels of Bcl-2 were reduced after eupafolin treatment. These findings suggest that eupafolin modulates the protein levels of Bid and Bcl-2, and this results in the release of cytochrome c from mitochondria.20397191
BIDBH3 interacting domain death agonist637Cervix AdenocarcinomaHeLa----Eupafolin decreased the cytosolic levels of pro-apoptotic Bid, but increased tBid mitochondrial levels. These findings suggest that eupafolin modulates the protein levels of Bid and this results in release of cytochrome c from mitochondria.20397191
CASP3caspase 3, apoptosis-related cysteine peptidase836Cervix AdenocarcinomaHeLa----The cleavage form of caspases-3, was found to increase in a dose-dependent manner after eupafolin treatment, and these inductions were accompanied by dose-dependent increases in the cleavages of their substrates (PARP and lamin A/C). caspase-3 activation was dectated at 12 h after treatment of eupafolin.20397191
CASP6caspase 6, apoptosis-related cysteine peptidase 839Cervix AdenocarcinomaHeLa----The cleavage form of caspases-6 was found to increase in a dose-dependent manner after eupafolin treatment, and these inductions were accompanied by dose-dependent increases in the cleavages of their substrates (PARP and lamin A/C).20397191
CASP7caspase 7, apoptosis-related cysteine peptidase840Cervix AdenocarcinomaHeLa----The cleavage form of caspases-7 was found to increase in a dose-dependent manner after eupafolin treatment, and these inductions were accompanied by dose-dependent increases in the cleavages of their substrates (PARP and lamin A/C).20397191
CASP8caspase 8, apoptosis-related cysteine peptidase841Cervix AdenocarcinomaHeLa----Eupafolin was found to induce the dose-dependent activations of initiator caspases, including caspase-8.The activations of this caspase was demonstrated by decreases in the levels of procaspase form and the appearances of their corresponding cleaved bands. Caspase-8 activation was only dectated at 24-48h after treatment of eupafolin.20397191
CASP9caspase 9, apoptosis-related cysteine peptidase842Cervix AdenocarcinomaHeLa----Eupafolin was found to induce the dose-dependent activations of initiator caspases, including caspase9. The activations of this caspase was demonstrated by decrease in the levels of procaspase forms and the appearances of their corresponding cleaved bands. Caspase-9 activation was detected at 3 h after eupafolin treatment and peaked at 48 .20397191
CYCScytochrome c54205Cervix AdenocarcinomaHeLa----Levels of cytosolic cytochrome c and Apaf-1 were elevated by eupafolin in HeLa cells, whereas XIAP levels decreased, and thus suggesting the involvement of the mitochondrial pathway in eupafolin-induced apoptosis.Cytosolic cytochrome c activates procaspase-9 by binding to Apaf-1, which leads to caspase-9 activation and the subsequent activations of downstream executioner caspases (caspases-3, -6, and -7).20397191

 






Copyright © 2012. Subhash M. Agagrwal. All Rights Reserved.