Two layered prediction of inhibitory efficacy of Allele specific siRNA for fully complementary mutant allele by ASPsiPred-SVM and wild-type allele with one-mismatch by ASPsiPred-matrix



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>Wild
GTGAGGAGGCGCGTTGAAAATATTGAGGCGCGTTGAAATTGTGAGGAGGCGTGAGG
>Mutant
GTGAGGAGGCGCGTTGAAAATAgTGAGGCGCGTTGAAATTGTGAGGAGGCGTGAGG