Instructions

BcePred Prediction Server

The BcePred server predicts B cell epitopes based on physico-chemical properties of amino acids.

Bcepred has been tested on B cell epitope database (BCIPEP), and will therefore presumbly have better performance for prediction of B cell epitope of the antigen. Bcepred can predict continuous B cell epitopes.

B-cell epitope plays an important role in humoral response and also in synthetic vaccine design. Identified properties of B cell epitope includes hydrophilicity, flexibility/mobility, accessibility, polarity, exposed surface and turns. Quantification of these properties is determined by assigning a value to each of the 20 natural amino acids. The server is able to predict epitopes with 58.7% accuracy using combined methods at a threshold of 2.38.

Instructions

(optional) Enter a name for the sequence.

Enter the sequence in the sequence window (with no header line), or give a file name.

The sequence must be written using the one letter amino acid code: `acdefghiklmnpqrstvwy' or `ACDEFGHIKLMNPQRSTVWY'.
Other letters will be converted to `X' and treated as unknown amino acids.
Other characters, such as whitespace and numbers, will simply be ignored.

Choose the output format: Graphical or Tabular, the default is Graphical.

Change the threshold: increase in the threshold results in better specificity, but worse sensitivity. At 2.37, it shows 58.3% accruacy with equal sensitivity and specificity using combined method.

Select the Physio-chemical properties: Hydrophilicity or Flexibility or Accessibility or Turns or Exposed surface or Polarity or Antegenic Propensity or combined Methods (All).For multiple selection use Ctrl key

Press the "Submit sequence" button.

A WWW page will return the results when the prediction is ready. Response time depends on system load.

BcePred Prediction Server

Instructions

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