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This server FDR4 is meant for the prediction of binding affinity of peptide binders in an antigenic sequence for a MHC class II allele HLA-DRB1*0401. Methods developed in the past can only predict whether a peptide is a binder or non-binder of this allele. Moreover, determining the binding core is also a problem in case of developing prediction methods for MHC class-II alleles. This server tries to overcome this problem and can predict both the binding core as well as the binding affinity of a peptide in an antigenic protein sequence.