Job Title:

The user can give job title or sequence name which will become 'subject' in the Email.

E-mail Address:

As sequence processing sometimes takes more time due to more number of input sequences,few stringent options selected which requires more computational time, it is advisable to give Email ID to run the process instead of waiting for a long time to publish result at the terminal itself.User can then stop the process and submit new job.All the job result will be mailed to him.

Protein Sequence:

Protein sequence(s) can be pasted directly in the box provided only in fasta format. Input can also be given from the file by using 'browse' button. Ideally the input should only be from one source either through box or file but not both.The server also has the capability to ignore all the non-standard characters such as *%!@$% etc present in the input sequence.

Prediction Approach:

User can use only one terminus method (Binary pattern,composition profile and PSSM) in SVM for their prediction at a time. Server also provides a number of options including selection of threshold for SVM and selection of the different terminals.

NAGsite is a web-server specially trained for the NAG interacting residues. The prediction is based on the basis of binary pattern of 17 window motif of amino acid sequence by using support vector machines (SVM). The prediction result will be displayed on web browser, PSSM based take some so user summit their mail-id result will be send by mail.