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Total number of Results for TOPO cloning are 31
Results from 0 - 25




Color Notation for result Interpretation

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Representing point mutations

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PAM sequence

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Target sequence

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Insertions

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Deletions



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CrisprID	Organisms	Gene/Locus	Uniprot	Pathway	Target sequence	Target/Mutant	Modifications length	Genetic Modifications	Location	Cell lines	Assay	PMID
Crispr0439	Homo sapiens	Casp8	Q14790		GCTCTTCCGAATTAATAGACTGG	GCTCTTCCGAATTAATA- - - G - - - - ACTGG GCTCTTCCGAATTAATAACAGAAAAACTGG	7	Insertion	Exon1	U251MG	TOPO cloning	24442637
Crispr0440	Homo sapiens	Casp8	Q14790		GCTCTTCCGAATTAATAGACTGG	GCTCTTCCGAATTAATA- - - G - - - - ACTGG GCTCTTCCGA -- - - - - - - - - G - - - - - - - - - -	D14	Deletion	Exon1	U251MG	TOPO cloning	24442637
Crispr0441	Homo sapiens	Casp8	Q14790		GCTCTTCCGAATTAATAGACTGG	GCTCTTCCGAATTAATAGACTGGT GCTCTTCCGA - - - - - - - - - - - - - -	D14	Deletion	Exon1	U251MG	TOPO cloning	24442637
Crispr0442	Homo sapiens	FAT1	Q14517	NA	TCCACCAAGGTCTTGGTGAAAGTC	CCA - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - AGGTCTTGGTGAAAGTC TGCTTTGCAGATTCTACAGAAAGACTGTTTCCAAACTGATCTATCAAGAGAATGGTTCAACTCTGTGGGGTGAATCACACAAGGTCTTGGTGAAAGTCTTA GGAAATCACACAAGGTCTTGGTGAAAGTC	82	Insertion	Exon1	U251MG	TOPO cloning	24442637
Crispr0443	Homo sapiens	FAT1	Q14517	NA	TCCACCAAGGTCTTGGTGAAAGTC	TCCACCAAGGTCTTGGTGAAAGTC TC - - - - - - - - - - TTGGTGAAAGTC	D10	Deletion	Exon1	U251MG	TOPO cloning	24442637
Crispr0444	Homo sapiens	FAT1	Q14517	NA	TCCACCAAGGTCTTGGTGAAAGTC	TCCACCAAGGTCTTGGTGAAAGTC TCCACCA - GGTCTTGGTGAAAGTC	D1	Deletion	Exon1	U251MG	TOPO cloning	24442637
Crispr3022	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCT - TTGT - TCAGGACACTTTTCC	1	insertion	NA	CHO-KI	TOPO cloning	24827782
Crispr3023	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCT - TTGT - TCAGGACACTTTTCC	1	insertion	NA	CHO-KI	TOPO cloning	24827782
Crispr3024	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCT - TTGT - TCAGGACACTTTTCC	1	insertion	NA	CHO-KI	TOPO cloning	24827782
Crispr3025	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCTCT - GT - TCAGGACACTTTTCC	1	insertion	NA	CHO-KI	TOPO cloning	24827782
Crispr3026	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCT - - - GTGTCAGGACACTTTTCC	D1,+1	Indels	NA	CHO-KI	TOPO cloning	24827782
Crispr3027	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCT - - - GT - TCAGGACACTTTTCC	D1	Deletion	NA	CHO-KI	TOPO cloning	24827782
Crispr3028	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC AC - - - - - GT - TCAGGACACTTTTCC	D3	Deletion	NA	CHO-KI	TOPO cloning	24827782
Crispr3029	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCC - - - - - - - CAGGACACTTTTCC	S1,D4	Deletion/Substitution	NA	CHO-KI	TOPO cloning	24827782
Crispr3030	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC AC - - - - - - - - - - - - - - - ACTTTTCC	D12	Deletion	NA	CHO-KI	TOPO cloning	24827782
Crispr3031	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC AC - - - - - - - - - - - - - - - - - TTTTCC	D13	Deletion	NA	CHO-KI	TOPO cloning	24827782
Crispr3032	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	TTCCAAATCATCCCACCT - T - GT -TCAGGACACTTTTCC TTCC - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - -- -	D32	Deletion	NA	CHO-KI	TOPO cloning	24827782
Crispr3033	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	TTCCAAATCATCCCACCT - T - GT -TCAGGACACTTTTCC - - - - - - - - - - - - -- - - - - - - - - - T - TCAGGACACTTTTCC	D20	Deletion	NA	CHO-KI	TOPO cloning	24827782
Crispr3034	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCT - T - GT - TCAGGACACTTTTCC	WT	WT	NA	CHO-KI	TOPO cloning	24827782
Crispr3035	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCT - T - GT - TCAGGACACTTTTCC	WT	WT	NA	CHO-KI	TOPO cloning	24827782
Crispr3036	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCT - T - GT - TCAGGACACTTTTCC	WT	WT	NA	CHO-KI	TOPO cloning	24827782
Crispr3037	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCT - T - GT - TCAGGACACTTTTCC	WT	WT	NA	CHO-KI	TOPO cloning	24827782
Crispr3038	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCT - T - GT - TCAGGACACTTTTCC	WT	WT	NA	CHO-KI	TOPO cloning	24827782
Crispr3039	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCT - T - GT - TCAGGACACTTTTCC	WT	WT	NA	CHO-KI	TOPO cloning	24827782
Crispr3040	Cricetulus griseus	C1GAIT1C1	G3IPT0		ACCTTGTTCAGGACACTTTTCC	ACCT - T - GT -TCAGGACACTTTTCC ACCT - T - GT - TCAGGACACTTTTCC	WT	WT	NA	CHO-KI	TOPO cloning	24827782



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